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|Antigen||Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) Antibodies|
|Reactivity||Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis Alternatives|
|Conjugate||This CTNNB1 antibody is un-conjugated Alternatives|
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-CTNNB1 AntibodyTarget Details CTNNB1 Application Details Handling Images
|Specificity||Recognizes human b-catenin. Species cross-reactivity: chicken, mouse, rat and Xenopus.|
A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein.
Type of Immunogen: Synthetic peptide
|Plasmids, Primers & others|
Target Details CTNNB1Product Details anti-CTNNB1 Antibody Application Details Handling Images back to top
|Alternative Name||CTNNB1 / Beta Catenin (CTNNB1 Antibody Abstract)|
Name/Gene ID: CTNNB1
Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin
|Pathways||WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2|
Application DetailsProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Handling Images back to top
Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (2.5 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot after IP: 1-2 μg/mL. Cross-reactivity with a ~100kD protein of unknown identity is sometimes observed. Immunoprecipitation: 5-10 μg. Specifically immunoprecipitate the ~92kD b-Catenin protein from native cell lysates. Positive control: HeLa and A431 lysates.
Target Species of Antibody: Human
|Restrictions||For Research Use only|
HandlingProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Images back to top
|Buffer||PBS, pH 7.4, 0.1 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
ImagesProduct Details anti-CTNNB1 Antibody Target Details CTNNB1 Application Details Handling back to top