anti-Human Phospholipase C beta 4 antibody for Western Blotting
Recommended Phospholipase C beta 4 Antibody (supplied by: Log in to see )
- Antigen
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Phospholipase C beta 4 (PLCb4) Antibodies
show synonyms for this antigen- ARCND2
- PI-PLC
- A930039J07Rik
- AI854601
- C230058B11Rik
- Beta4aa
- RATBETA4AA
- phospholipase C beta 4
- phospholipase C, beta 4
- PLCB4
- Plcb4
- Epitope
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AA 752-961
Alternatives - Reactivity
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Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus)
Alternatives - Host
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Mouse
Alternatives - Clonality
- Application
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BioImaging (BI), Immunofluorescence (IF), Western Blotting (WB)
Alternatives - Options
- Supplier
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- Supplier Product No.
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 3.283734 | ABIN968900 | IF WB | Mouse | IgG1 | AA 752-961 | Log in to see | 56-Phospholipase Cbeta4 | 4 | ||
| 3.283734 | ABIN5014111 | ICC IHC WB | Rabbit | IgG | AA 2-250 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN5515611 | WB | Rabbit | Middle Region | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN2149229 | ELISA WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN1856286 | ELISA WB | Rabbit | Log in to see | Polyclonal | 0 |
General |
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|---|---|
| Antigen | Phospholipase C beta 4 (PLCb4) Antibodies |
| Epitope | AA 752-961 Alternatives |
| Reactivity | Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus) Alternatives |
| Host | Mouse Alternatives |
| Clonality (Clone) | |
| Application |
BioImaging (BI), Immunofluorescence (IF), Western Blotting (WB)
Alternatives
|
|
4 references available |
| Supplier | Log in to see |
Product Details anti-Phospholipase C beta 4 AntibodyTarget Details Phospholipase C beta 4 Application Details Handling References for anti-Phospholipase C beta 4 antibody (ABIN968583) Images |
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| Cross-Reactivity | Rat (Rattus), Mouse (Murine), Fruit Fly (Drosophila melanogaster) |
| Characteristics |
1. Please refer to us for technical protocols. 2. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 3. Alexa Fluor is a registered trademark of Molecular Probes, Inc., Eugene, OR. 4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. 5. Source of all serum proteins is from USDA inspected abattoirs located in the United States. |
| Purification | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. |
| Immunogen | Human Phospholipase Cbeta4 aa. 752-961 |
| Clone | 56-Phospholipase Cbeta4 |
| Isotype | IgG1 |
Target Details Phospholipase C beta 4Product Details anti-Phospholipase C beta 4 Antibody Application Details Handling References for anti-Phospholipase C beta 4 antibody (ABIN968583) Images back to top |
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| Antigen | |
| Alternative Name | Phospholipase C beta 4 (PLCb4 Antibody Abstract) |
| Background |
Phospholipase C (PLC) hydrolyzes inositol phospholipids into diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Multiple distinct PLC isoenzymes have been identified and divided into three structural types: alpha, beta, and gamma. This classification is based primarily on the location of the conserved X and Y domains, whose structural integrity is essential for a functional catalytic core. The activation of PLCbeta isoenzymes is uniquely regulated by G protein subunits, while PLCgamma is activated following phosphorylation by protein tyrosine kinases. The beta subfamily of PLC consists of at least four members: beta1, beta2, beta3, and beta4. PLCbeta4 differs from the other members in that it is not activated by G protein betagamma subunits, it is not found in the liver or kidney, and it is inhibited by ribonucleotides. Various isoforms of PLbetaC4 result from alternative splicing or proteolytic cleavage. PLCbeta4 is expressed in retina and brain and knockout mice display ataxia and abnormalities in metabotropic glutamate receptor function in the cerebellum. Thus, PLCbeta4 is primarily found in neuronal tissues where it is thought to be important in neurotransmitter signaling pathways. Synonyms: PLCbeta4 |
| Molecular Weight | 130 kDa |
| Pathways | WNT Signaling, Thyroid Hormone Synthesis, G-protein mediated Events |
Application DetailsProduct Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Handling References for anti-Phospholipase C beta 4 antibody (ABIN968583) Images back to top |
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| Application Notes |
Bioimaging: Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS. Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS. |
| Comment |
Related Products: ABIN967389 |
| Restrictions | For Research Use only |
HandlingProduct Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details References for anti-Phospholipase C beta 4 antibody (ABIN968583) Images back to top |
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| Format | Liquid |
| Concentration | 250 μg/mL |
| Buffer | Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide. |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Storage | -20 °C |
| Storage Comment | Store undiluted at -20°C. |
References for anti-Phospholipase C beta 4 antibody (ABIN968583)Product Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details Handling Images back to top |
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| Product cited in: |
Kano, Hashimoto, Watanabe, Kurihara, Offermanns, Jiang, Wu, Jun, Shin, Inoue, Simon, Wu: "Phospholipase cbeta4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 26, pp. 15724-9, 1999 Kim, Jun, Lee, Kang, Min, Kim, Ryu, Suh, Shin: "Phospholipase C isozymes selectively couple to specific neurotransmitter receptors." in: Nature, Vol. 389, Issue 6648, pp. 290-3, 1997 Alvarez, Ghalayini, Xu, Hardcastle, Bhattacharya, Rao, Pettenati, Anderson, Baehr: "cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)." in: Genomics, Vol. 29, Issue 1, pp. 53-61, 1996 Lee, Park, Lee, Kim, Rhee: "Purification, molecular cloning, and sequencing of phospholipase C-beta 4." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21318-27, 1993 |
ImagesProduct Details anti-Phospholipase C beta 4 Antibody Target Details Phospholipase C beta 4 Application Details Handling References for anti-Phospholipase C beta 4 antibody (ABIN968583) back to top |
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| Supplier Images |
Western blot analysis of Phospholipase Cbeta4 on a rat pituitary lysate (left). Lane ...
Immunofluorescent staining of C6 cells (Rat glioma, ATCC CCL-107) (right). Cells were...
anti-Phospholipase C beta 4 (PLCb4) (AA 752-961) antibody (Image 3)
anti-Phospholipase C beta 4 (PLCb4) (AA 752-961) antibody (Image 4)
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