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|Antigen||Rhodopsin (RHO) Antibodies|
|Reactivity||Human, Rat (Rattus), Mouse (Murine) Alternatives|
|Conjugate||This Rhodopsin antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
Product Details anti-Rhodopsin AntibodyTarget Details Rhodopsin Application Details Handling References for anti-Rhodopsin antibody (ABIN350720) Images
|Specificity||Specific for RhO family.|
|Immunogen||A synthetic peptide from human Rho-related GTP-binding proteins (RhOA, RhOB, RhOC, RhOF, RhOQ) and RAC1, RAC2, RAC3 and CDC42 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many other species.|
|Plasmids, Primers & others|
Target Details RhodopsinProduct Details anti-Rhodopsin Antibody Application Details Handling References for anti-Rhodopsin antibody (ABIN350720) Images back to top
|Background||The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Also known as: Rho-related GTP-binding protein RhOA, RhOB, RhOC, RhOF, RhOQ, RAC1, RAC2, RAC3, CDC42.|
|Pathways||WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction|
Application DetailsProduct Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling References for anti-Rhodopsin antibody (ABIN350720) Images back to top
A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details References for anti-Rhodopsin antibody (ABIN350720) Images back to top
|Reconstitution||Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.|
|Handling Advice||Avoid freeze and thaw cycles.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Expiry Date||12 months|
References for anti-Rhodopsin antibody (ABIN350720)Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling Images back to top
Carninci, Kasukawa, Katayama, Gough, Frith, Maeda, Oyama, Ravasi, Lenhard, Wells, Kodzius, Shimokawa, Bajic, Brenner, Batalov, Forrest, Zavolan, Davis, Wilming, Aidinis, Allen, Ambesi-Impiombato et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005
Westmark, Bartleson, Malter: "RhoB mRNA is stabilized by HuR after UV light." in: Oncogene, Vol. 24, Issue 3, pp. 502-11, 2005
Nakamura, Asano, Shindo-Okada, Nishimura, Monden: "Cloning of the RhoB gene from the mouse genome and characterization of its promoter region." in: Biochemical and biophysical research communications, Vol. 226, Issue 3, pp. 688-94, 1996
Shinjo, Koland, Hart, Narasimhan, Johnson, Evans, Cerione: "Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 24, pp. 9853-7, 1991
Munemitsu, Innis, Clark, McCormick, Ullrich, Polakis: "Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42." in: Molecular and cellular biology, Vol. 10, Issue 11, pp. 5977-82, 1990
ImagesProduct Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin antibody (ABIN350720) back to top