anti-Mouse (Murine) Rhodopsin antibody for Western Blotting

Recommended Rhodopsin Antibody (supplied by: Log in to see )

Antigen
Rhodopsin (RHO) Antibodies
  • CSNBAD1
  • OPN2
  • RP4
  • RH1
  • Rh
  • fi06d11
  • wu:fi06d11
  • zfo2
  • zfrho
  • Noerg1
  • Opn2
  • Ops
  • opn2
  • rh1
  • rp4
  • xrho
  • PIGRHO1
  • ops
  • RDP1
  • RHO
  • rhodopsin
  • rhodopsin L homeolog
  • RHO
  • Rho
  • rho
  • rho.L
Reactivity
Human, Rat (Rattus), Mouse (Murine)
134
57
49
49
39
36
36
35
23
22
21
18
18
16
4
2
2
2
2
1
1
Host
Rabbit
217
70
5
1
Clonality
Polyclonal
Conjugate
This Rhodopsin antibody is un-conjugated
15
14
12
9
9
9
6
6
6
6
6
6
6
6
6
6
4
4
4
4
4
4
4
4
4
4
4
4
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
270
204
163
142
131
122
44
40
3
2
1
1
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Catalog No. ABIN350720
$ 454.67
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.755764 ABIN350721 IHC WB Rabbit IgG Log in to see Polyclonal 5
12.755764 ABIN350722 IHC WB Sheep Log in to see Polyclonal 5
12.755764 ABIN350723 IHC WB Sheep IgG Log in to see Polyclonal 5
12.302607 ABIN1532407 IHC ELISA WB Rabbit IgG AA 299-348 Log in to see Polyclonal 1
7.8026066 ABIN6257398 ELISA WB Rabbit IgG Log in to see Polyclonal 0
7.8026066 ABIN5535824 WB Rabbit Ig Fraction AA 310-339, C-Term Log in to see Polyclonal 0
7.8026066 ABIN1580458 ICC IF IHC WB Mouse IgG1 Log in to see B630 2
7 ABIN2888702 ELISA IHC IHC (p) WB Rabbit IgG AA 299-348 Log in to see Polyclonal 0
7 ABIN1960118 IF IHC IHC (p) WB Mouse IgG1, kappa Log in to see A531 0
7 ABIN1960117 ICC IF IHC IHC (p) WB Mouse IgG1, kappa Log in to see B630 0
4.8026066 ABIN2788258 WB Rabbit C-Term Log in to see Polyclonal 0
4.8026066 ABIN1853174 ELISA WB Rabbit Internal Region Log in to see Polyclonal 0
4 ABIN1103993 ICC IP IHC ELISA WB Mouse IgG1 N-Term Log in to see Rho 4D2 0
4 ABIN2451717 IF IHC IHC (p) WB Mouse IgG1 Log in to see RET-P1 0
1 ABIN1952432 ICC IP IHC ELISA WB Atto 488 Mouse IgG1 N-Term Log in to see 4D2 0
1 ABIN1952456 ICC IP IHC ELISA WB Atto 655 Mouse IgG1 N-Term Log in to see 4D2 0
1 ABIN1952468 ICC IP IHC ELISA WB Atto 700 Mouse IgG1 N-Term Log in to see 4D2 0
1 ABIN1952474 ICC IP IHC ELISA WB Biotin Mouse IgG1 N-Term Log in to see 4D2 0
1 ABIN1952492 ICC IP IHC ELISA WB FITC Mouse IgG1 N-Term Log in to see 4D2 0
1 ABIN1952533 ICC IP IHC ELISA WB Streptavidin Mouse IgG1 N-Term Log in to see 4D2 0

General

Antigen Rhodopsin (RHO) Antibodies
Reactivity Human, Rat (Rattus), Mouse (Murine)
(134), (57), (49), (49), (39), (36), (36), (35), (23), (22), (21), (18), (18), (16), (4), (2), (2), (2), (2), (1), (1)
Host Rabbit
(217), (70), (5), (1)
Clonality
Polyclonal
Conjugate This Rhodopsin antibody is un-conjugated
(15), (14), (12), (9), (9), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(270), (204), (163), (142), (131), (122), (44), (40), (3), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-Rhodopsin Antibody

Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin antibody (ABIN350720) Images
Specificity Specific for RhO family.
Purification Whole serum
Immunogen A synthetic peptide from human Rho-related GTP-binding proteins (RhOA, RhOB, RhOC, RhOF, RhOQ) and RAC1, RAC2, RAC3 and CDC42 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many other species.
Plasmids, Primers & others

Target Details Rhodopsin

Product Details anti-Rhodopsin Antibody Application Details Handling References for anti-Rhodopsin antibody (ABIN350720) Images back to top
Antigen
Target Type Chemical
Background The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Also known as: Rho-related GTP-binding protein RhOA, RhOB, RhOC, RhOF, RhOQ, RAC1, RAC2, RAC3, CDC42.
Pathways WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction

Application Details

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling References for anti-Rhodopsin antibody (ABIN350720) Images back to top
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only

Handling

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details References for anti-Rhodopsin antibody (ABIN350720) Images back to top
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months

References for anti-Rhodopsin antibody (ABIN350720)

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling Images back to top
Background publications

Carninci, Kasukawa, Katayama, Gough, Frith, Maeda, Oyama, Ravasi, Lenhard, Wells, Kodzius, Shimokawa, Bajic, Brenner, Batalov, Forrest, Zavolan, Davis, Wilming, Aidinis, Allen, Ambesi-Impiombato et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005

Westmark, Bartleson, Malter: "RhoB mRNA is stabilized by HuR after UV light." in: Oncogene, Vol. 24, Issue 3, pp. 502-11, 2005

Nakamura, Asano, Shindo-Okada, Nishimura, Monden: "Cloning of the RhoB gene from the mouse genome and characterization of its promoter region." in: Biochemical and biophysical research communications, Vol. 226, Issue 3, pp. 688-94, 1996

Shinjo, Koland, Hart, Narasimhan, Johnson, Evans, Cerione: "Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 24, pp. 9853-7, 1991

Munemitsu, Innis, Clark, McCormick, Ullrich, Polakis: "Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42." in: Molecular and cellular biology, Vol. 10, Issue 11, pp. 5977-82, 1990

Images

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin antibody (ABIN350720) back to top
Supplier Images
Western Blotting (WB) image for anti-Rhodopsin (RHO) antibody (ABIN350720) WB on rat brain lysate using Rabbit antibody to pan RhO (A, B, C, F, Q), RAC (1, 2, 3...
 image for anti-Rhodopsin (RHO) antibody (ABIN350720) IHC on rat midbrain using Rabbit antibody to pan RhO (A, B, C, F, Q), RAC (1, 2, 3) a...
 image for anti-Rhodopsin (RHO) antibody (ABIN350720) IF on 1 day- old mouse cortical cell culture using Rabbit antibody to pan RhO (A, B, ...
Immunofluorescence (IF) image for anti-Rhodopsin (RHO) antibody (ABIN350720) IF on 1 day- old mouse cortical cell culture using Rabbit antibody to pan RhO, RAC, C...