anti-Cow (Bovine) SFRP1 antibody for Enzyme Immunoassay

Recommended SFRP1 Antibody (supplied by: Log in to see )

Antigen
Secreted Frizzled-Related Protein 1 (SFRP1) Antibodies
  • SFRP1
  • XfrzA
  • frp
  • frp-1
  • frp1
  • frzA
  • sarp2
  • sfrp1
  • LOC100231868
  • FRP
  • FRP-1
  • FRP1
  • FrzA
  • SARP2
  • CSFRP1
  • 2210415K03Rik
  • AW011917
  • AW107218
  • AW742929
  • sFRP-1
  • secreted frizzled-related protein 1
  • Secreted frizzled-related protein 1
  • secreted frizzled-related protein 1-like
  • SFRP1
  • sfrp1
  • LOC100231868
  • Sfrp1
  • LOC100621622
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
97
42
36
10
5
4
3
2
1
1
Host
Rabbit
82
25
2
2
Clonality
Polyclonal
Conjugate
This SFRP1 antibody is un-conjugated
9
6
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
69
65
34
27
14
10
7
5
5
3
3
2
1
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Catalog No. ABIN5551728
$ 411.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN569588 EIA IHC (p) Goat C-Term Log in to see Polyclonal
1 ABIN303265 EIA IF IHC (p) WB Rabbit AA 43-54 Log in to see Polyclonal

General

Antigen Secreted Frizzled-Related Protein 1 (SFRP1) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(97), (42), (36), (10), (5), (4), (3), (2), (1), (1)
Host Rabbit
(82), (25), (2), (2)
Clonality
Polyclonal
Conjugate This SFRP1 antibody is un-conjugated
(9), (6), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(69), (65), (34), (27), (14), (10), (7), (5), (5), (3), (3), (2), (1)
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Product Details anti-SFRP1 Antibody

Target Details SFRP1 Application Details Handling Images
Specificity This affinity purified antibody is directed against Human SFRP protein. A BLAST analysis was used to suggest reactivity with this protein from Human based on 100 % homology for the immunogen sequence. Expect cross reactivity with SFRP1 from Mouse, Rat and Bovine sources as only a single amino acid residue change is found within the immunogen sequence (91 % positive by BLAST). Cross reactivity with SFRP1 homologues from other sources has not been determined.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat and Bovine.
Species reactivity (tested):Human.
Characteristics Concentration: 0.56 mg/mL (by UV absorbance at 280 nm)
Purification Immunoaffinity Chromatography
Immunogen Synthetic peptide corresponding to a 12 amino acid region of Human Sfrp1 protein
Isotype IgG

Target Details SFRP1

Product Details anti-SFRP1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name SFRP1 (SFRP1 Antibody Abstract)
Background SFRP1 (also known as FRP, FRP1, SARP2, Secreted Apoptosis-related Protein 2, Secreted Frizzledrelated Protein and Secreted frizzled-related protein 1) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.Synonyms: FRP, FRP-1, FRP1, SARP-2, SARP2, SFRP-1, Secreted apoptosis-related protein 2, Secreted frizzled-related protein 1
Molecular Weight 35.4 kDa (Predicted)
Gene ID 6422
NCBI Accession NP_003003
UniProt Q8N474
Research Area Organogenesis, Proteasome, Proteolysis / Ubiquitin, Wnt, Wnt Signaling
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-SFRP1 Antibody Target Details SFRP1 Handling Images back to top
Application Notes ELISA: 1: 5,000 - 1: 25,000. Immunohistochemistry on paraffin sections: 1: 800. Western blot: 1: 200 - 1: 2,000 (Expect a band approximately 37 kDa in size corresponding toSfrp1 in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-SFRP1 Antibody Target Details SFRP1 Application Details Images back to top
Concentration 0.56 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.