anti-Pig (Porcine) TLE2 antibody for Western Blotting

Recommended TLE2 Antibody (supplied by: Log in to see )

Antigen
Transducin-Like Enhancer of Split 2 (E(sp1) Homolog, Drosophila) (TLE2) Antibodies
  • TLE2
  • ESG
  • ESG2
  • GRG2
  • Grg2
  • mKIAA4188
  • transducin like enhancer of split 2
  • transducin-like enhancer of split 2
  • TLE2
  • Tle2
Epitope
N-Term
12
11
8
5
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
39
21
3
1
1
1
1
Host
Rabbit
47
6
1
Clonality
Polyclonal
Conjugate
This TLE2 antibody is un-conjugated
2
2
2
2
2
2
Application
Western Blotting (WB)
52
29
10
5
5
4
3
2
1
1
Options
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Available images

Catalog No. ABIN2779582
$ 289.00
Plus shipping costs $45.00

General

Antigen Transducin-Like Enhancer of Split 2 (E(sp1) Homolog, Drosophila) (TLE2) Antibodies
Epitope N-Term
(12), (11), (8), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(39), (21), (3), (1), (1), (1), (1)
Host Rabbit
(47), (6), (1)
Clonality
Polyclonal
Conjugate This TLE2 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(52), (29), (10), (5), (5), (4), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-TLE2 Antibody

Target Details TLE2 Application Details Handling References for anti-TLE2 antibody (ABIN2779582) Images
Blocking Peptide Blocking peptide for this product available: ABIN985126
Sequence VEEERPSGPG GGGKQRADEK EPSGPYESDE DKSDYNLVVD EDQPSEPPSP
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 85%, Pig: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against TLE2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TLE2
Plasmids, Primers & others

Target Details TLE2

Product Details anti-TLE2 Antibody Application Details Handling References for anti-TLE2 antibody (ABIN2779582) Images back to top
Antigen
Alternative Name TLE2 (TLE2 Antibody Abstract)
Background TLE2 belongs to the WD repeat Groucho/TLE family. It contains 6 WD repeats. TLE2 is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
Alias Symbols: ESG, ESG2, FLJ41188, GRG2
Protein Interaction Partner: UBC, SUMO1, ORF50, LEF1, BARHL1, TLX3, VENTX, CUL4A, CUL4B, PAX9, MSX2, MSX1, FOXA3, EMX2, PRDM1, H3F3B, TLE1, UTY, HMGB1, RUNX1, TLE2, HES1,
Protein Size: 743
Molecular Weight 80 kDa
Gene ID 7089
NCBI Accession NM_003260, NP_003251
UniProt Q04725
Pathways WNT Signaling

Application Details

Product Details anti-TLE2 Antibody Target Details TLE2 Handling References for anti-TLE2 antibody (ABIN2779582) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 743 AA

Restrictions For Research Use only

Handling

Product Details anti-TLE2 Antibody Target Details TLE2 Application Details References for anti-TLE2 antibody (ABIN2779582) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TLE2 antibody (ABIN2779582)

Product Details anti-TLE2 Antibody Target Details TLE2 Application Details Handling Images back to top
Background publications

Higa, Wu, Ye, Kobayashi, Sun, Zhang: "CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation." in: Nature cell biology, Vol. 8, Issue 11, pp. 1277-83, 2006

Images

Product Details anti-TLE2 Antibody Target Details TLE2 Application Details Handling References for anti-TLE2 antibody (ABIN2779582) back to top
Supplier Images
Western Blotting (WB) image for anti-Transducin-Like Enhancer of Split 2 (E(sp1) Homolog, Drosophila) (TLE2) (N-Term) antibody (ABIN2779582) WB Suggested Anti-TLE2 Antibody Titration: 0.2-1 ug/ml Positive Control: Transfect...