c-MYC antibody (C-Term) (HRP)

Catalog No. ABIN119683

This Mouse Monoclonal antibody specifically detects c-MYC in WB, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for c-MYC antibody (C-Term) (HRP) (ABIN119683)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MYC antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 9-00E-010

Product Details anti-c-MYC Antibody (HRP)

Binding Specificity: C-Term

Sequence: AEEQKLISEE DLL

Specificity: This antibody detects the 62 kDa c-myc gene product. SM1863HRP may also be used to detect the commonly used c-myc 'tag'.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Synthetic peptide sequence corresponding to the C-terminal region of Human c-myc.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/100-1/500. Western blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining and does not requireantigen retrieval using heat treatment prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.01 % Thimerosal, HRP Stabilizer

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: C-Myc

Background: C-myc gene product is involved in the regulation of the cell cycle and cell growth. It is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag

Gene ID: 4609

NCBI Accession: NP_002458

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect