STAT3 antibody (phosphorylated)

Catalog No. ABIN126899

Product Details anti-STAT3 Antibody

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Binding Specificity: phosphorylated

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody specifically recognizes STAT3 phosphorylated at Ser 727. The antibody does not crossreact with the non-phosphorylated form of STAT3 nor with unrelated serine-phosphorylated proteins.

Characteristics: Synonyms: STAT-3, Acute-phase response factor, APRF, Signal transducer and activator oftranscription 3

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjuagted to KLH

Clone: 23G5

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: STAT3 (STAT3 Products)

Synonyms: 1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody

Background: The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. Besides tyrosine phosphorylation, STAT3 activity is regulated by serine phosphorylation at serine 727. Recent reports indicate that both MAP kinase and SAP kinase induce phosphorylaton at serine 727.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3

Gene ID: 6774

UniProt: P40763

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor