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RIPK1 antibody (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1)

Details for Product anti-RIPK1 Antibody No. ABIN127073, Supplier: Log in to see
Antigen
  • C9orf12
  • RGD1311271
  • rIpk1
  • RIP
  • RIP1
  • D330015H01Rik
  • Rinp
  • Rip1
  • 2400006N03Rik
  • RP23-83I13.5
  • Rip
Alternatives
anti-Human RIPK1 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
107
30
30
6
2
2
Host
Rabbit
79
25
3
Clonality
Polyclonal
Conjugate
This RIPK1 antibody is un-conjugated
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
86
37
36
22
19
12
5
2
1
Supplier
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Immunogen Synthetic peptide surrounding amino acid 645 of Human RIP
Specificity The antibody detects 74 kDa RIP in Western blot analysis.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification Affinity Chromatography.
Alternative Name RIP (RIPK1 Antibody Abstract)
Background RIP (Receptor Interacting Protein) is a 74 kDa Ser/Thr kinase which interacts with CD95 (Fas/Apo1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID, and TRAF2. RIP contains an N-terminal region with homology to protein kinase, an intermediate domain capable of association with MAPKKK and a C-terminal region containing an intracellular death domain motif. RIP activates both p38 MAP kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.Synonyms: Cell death protein RIP, RIPK1, Receptor-Interacting Protein, Receptor-interacting serine/threonine-protein kinase 1, Serine/threonine-protein kinase RIP
Gene ID 8737
NCBI Accession NP_003795
UniProt Q13546
Research Area Cancer, Apoptosis/Necrosis
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling
Application Notes Western Blot: 2-6 μg/mL. Immunoprecipitation. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS, pH 7.2, containing 30 % Glycerol, 0.5 % BSA as stabilizer and 0.01 % Thimerosal as preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C/-80 °C
Storage Comment Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for long term storage.
Background publications Hsu, Huang, Shu et al.: "TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex." in: Immunity, Vol. 4, Issue 4, pp. 387-96, 1996 (PubMed).

Stanger, Leder, Lee et al.: "RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death." in: Cell, Vol. 81, Issue 4, pp. 513-23, 1995 (PubMed).