Rabbit Anti-Akt (Phospho-Ser473) detects endogenous human, mouse, and rat Akt1, Akt2 and Akt3, phosphorylated at serine 473, serine 474, and serine 472, respectively.
Purification
GenScript Rabbit Anti-Akt (Phospho-Ser473) Polyclonal Antibody is purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide and cross-adsorbed with the corresponding non-phosphopeptide.
Immunogen
Synthesized phosphopeptide derived from human Akt around the phosphorylation site of pSer473 (Q-F-SP-Y-S)
AKT1
Reactivity: Human
WB, IHC, IF, IP
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.5-2.0 µg/mL Other applications: user-optimized
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage
4 °C/-20 °C
Storage Comment
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Azakir, Angers: "Reciprocal regulation of the ubiquitin ligase Itch and the epidermal growth factor receptor signaling." in: Cellular signalling, Vol. 21, Issue 8, pp. 1326-36, (2009) (PubMed).
Akt, also known as protein kinase B (PKB), a serine/ threonine kinase, is a critical enzyme in several signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. Akt is activated following its phosphorylation at two regulatory residues. Phosphorylation of threonine on the kinase domain, catalyzed by PDK1, is essential for Akt activation. Akt activity is augmented approximately ten fold by phosphorylation at the serine on the hydrophobic motif by PDK2. Phosphorylation of Thr308 and Ser473 activates Akt alpha. Phosphorylation at Thr309 and Ser474 on Akt beta1 and beta2, and on Thr305 on Akt gamma results in their activation. Akt promotes cell survival by inhibiting apoptosis by phosphorylating and inactivating several targets, including bad, forkhead transcription factors, c-Raf and caspase-9. The activation of Akt is negatively regulated by PTEN, a PIP3-specific phosphatase, and SHIP, a SH2-domain containing inositol 5-phosphatase.Rabbit Anti-Akt (Phospho-Ser473) Polyclonal Antibody is developed in rabbit using a synthetic phosphopeptide corresponding to residues surrounding S473 of human, rat, and mouse Akt alpha, also known as Protein Kinase B alpha (PKBalpha), Akt1, and RACalpha. In addition, this phosphopeptide is identical to the sequence surrounding S474 of human, rat, and mouse Akt beta (Akt2) and S472 of human and mouse Akt gamma (Akt3).