Forkhead Box O3 (FOXO3) (C-Term) antibody

Details for Product No. ABIN185248
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Antigen
Synonyms FOXO3, FKHRL1, FOXO3A, zgc:92176, wu:fb50g02, wu:fi33f07, foxo3a, xfoxo3, foxO3, foxO, AF6q21, FKHRL1P2, FOXO2, 1110048B16Rik, 2010203A17Rik, C76856, Fkhr2, Foxo3a, FoxO3A, Fkhrl1, FOXO-3
Epitope
C-Term
(39), (27), (27), (17), (12), (12), (12), (7), (7), (6), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(210), (112), (96), (57), (54), (53), (50), (4), (1), (1), (1)
Host
Goat
(164), (38), (10), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(152), (93), (80), (62), (48), (40), (25), (8), (2)
Pubmed 1 reference available
Quantity 100 µg
Options
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Catalog No. ABIN185248
374.27 $
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Immunogen Peptide with sequence C-GAKQASSQSWVPG, from the C Terminus of the protein sequence according to NP_001446.1.
Specificity Both variants represent identical protein (NP_001446.1and NP_963853.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXO3A / FKHRL1
Background Synonyms: FOXO3A, FKHRL1, AF6q21, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, forkhead box O3A, forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1, FOXO3, forkhead box O3, FOXO2, forkhead homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
Gene ID 2309, 56484
NCBI Accession NP_001446
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90 kDa band observed in human Brain and Heart lysates (calculated MW of 71.3 kDa according to NP_001446 and NP_963853). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Forkhead Box O3 (FOXO3) (C-Term) antibody ABIN185248 staining (0.5µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Brunet, Bonni, Zigmond et al.: "Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor." in: Cell, Vol. 96, Issue 6, pp. 857-68, 1999 (PubMed).

Validation Images
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