SDHB antibody (C-Term)
-
- Target See all SDHB Antibodies
- SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))
-
Binding Specificity
- C-Term
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This SDHB antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- SDHB
- Sequence
- ATYKEKKASV
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-ATYKEKKASV, from the C Terminus of the protein sequence according to NP_002991.2.
- Isotype
- IgG
- Top Product
- Discover our top product SDHB Primary Antibody
-
-
- Application Notes
-
Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 6-8 μg/mL.
Western Blot: Approx 28-30 kDa band observed in lysates of cell lines HEK293, Jurkat and HeLa (calculated MW of 31.6 kDa according to NP_002991.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:8000.
- Comment
-
Immunofluorescence: Strong expression of the protein seen in the plasm membrane/ cytoplasm of A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentr
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- Target
- SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))
- Alternative Name
- SDHB (SDHB Products)
- Synonyms
- succinate dehydrogenase 2-3 antibody, CG3283 antibody, Dmel\\CG3283 antibody, Ip antibody, SDH antibody, SDH-IP antibody, SDH-Ip antibody, SDHb antibody, sdhB antibody, CWS2 antibody, IP antibody, PGL4 antibody, SDH1 antibody, SDH2 antibody, SDHIP antibody, 0710008N11Rik antibody, fj46d06 antibody, wu:fj46d06 antibody, succinate dehydrogenase complex iron sulfur subunit B antibody, succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3 antibody, Succinate dehydrogenase, subunit B (iron-sulfur) antibody, succinate dehydrogenase complex subunit B, iron sulfur (Ip) antibody, succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody, succinate dehydrogenase complex subunit B, iron sulfur (Ip) S homeolog antibody, SDHB antibody, SDH2-3 antibody, SdhB antibody, sdhb antibody, Sdhb antibody, sdhb.S antibody
- Background
- SDHB, IP, SDH, SDH1, SDHIP, succinate dehydrogenase complex, subunit B, iron sulfur (Ip), OTTHUMP00000044624, iron-sulfur subunit, FLJ92337, PGL4, SDH2, SDHIP
- Gene ID
- 6390
- NCBI Accession
- NP_002991
-