P21-Activated Kinase 1 (PAK1) (Internal Region) antibody

Details for Product No. ABIN185685
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Antigen
Synonyms X-PAK1, XPak1, pak, pak-1, pakalpha, PAKalpha, AW045634, PAK-1, Paka
Epitope
Internal Region
(38), (17), (14), (12), (12), (10), (8), (8), (6), (5), (5), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(227), (127), (126), (38), (12), (1)
Host
Goat
(216), (10), (4), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(167), (89), (88), (51), (40), (36), (19), (14), (14), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185685
374.27 $
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  • +1 404 474 4654
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Immunogen Peptide with sequence C-NTEKQKKKPKMSDE, from the internal region of the protein sequence according to NP_002567.3.
Cross-Reactivity Dog (Canine)
Cross-Reactivity (Details) No cross-reactivity exptected with PAK3
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PAK1
Background Synonyms: PAK1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), MGC130000, MGC130001, PAKalpha, p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
Gene ID 5058, 18479, 29431
NCBI Accession NP_002567
Research Area Cancer, Apoptosis/Necrosis, Kinases/Phosphatases
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 70 kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 60.6 kDa according to NP_002567.3). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-P21-Activated Kinase 1 (PAK1) (Internal Region) antibody ABIN185685 (1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Zhao, Ma, Calon et al.: "Role of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease." in: Nature neuroscience, Vol. 9, Issue 2, pp. 234-42, 2006 (PubMed).

Validation Images
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