MGMT antibody

Catalog No. ABIN1882266

Product Details anti-MGMT Antibody

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged MGMT protein was used to produced this monoclonal antibody.

Clone: 888CT22-2-4

Isotype: IgG1

Application Details

Application Notes: IF: 1:25. WB: 1:1000. IHC: 1:100. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-MGMT antibody (ABIN1882266)

Edara, Goodtzova, Pegg: "The role of tyrosine-158 in O6-alkylguanine-DNA alkyltransferase activity." in: Carcinogenesis, Vol. 16, Issue 7, pp. 1637-42, (1995) (PubMed).

Tano, Shiota, Collier, Foote, Mitra: "Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 2, pp. 686-90, (1990) (PubMed).

Rydberg, Spurr, Karran: "cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells." in: The Journal of biological chemistry, Vol. 265, Issue 16, pp. 9563-9, (1990) (PubMed).

Koike, Maki, Takeya, Hayakawa, Sekiguchi: "Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase." in: The Journal of biological chemistry, Vol. 265, Issue 25, pp. 14754-62, (1990) (PubMed).

Hayakawa, Koike, Sekiguchi: "Expression and cloning of complementary DNA for a human enzyme that repairs O6-methylguanine in DNA." in: Journal of molecular biology, Vol. 213, Issue 4, pp. 739-47, (1990) (PubMed).

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT (MGMT Products)

Synonyms: AGT antibody, AI267024 antibody, Agat antibody, O-6-methylguanine-DNA methyltransferase antibody, MGMT antibody, Mgmt antibody

Background: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Molecular Weight: 21646

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus