This Phospholipase C gamma 1 antibody is un-conjugated
Application
Western Blotting (WB)
Specificity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. PLCγ1 antibody detects endogenous levels of total PLCγ1 protein.
Purification
Immunoaffinity chromatography.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human PLCgamma1 around the phosphorylation site of tyrosine 783 (G-F-Yp-V-E).
PLCG1
Reactivity: Mouse, Rat
WB, ELISA, IF (p), IHC (p), IF (cc), FACS, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Suitable for use in Western blot (1: 500-1: 1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody (in aliquots) at -20 °C.
Target
Phospholipase C gamma 1 (PLCG1)
(phospholipase C, gamma 1 (PLCG1))