CD22 antibody (PE)

Catalog No. ABIN2144831

Product Details anti-CD22 Antibody (PE)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD22 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Characteristics: HIB22 reacts with CD22, a 140 kDa B-cell specific molecule, expressed in the cytoplasm of all B lymphocytes and on the cell surface of only mature B cells. CD22 antigen is present in the most B-cell leukemias and lymphomas but not T-cell leukemias. In contrast with CD10, CD19 and CD20 antigen, CD22 antigen is still present on lymphoplasmacytoid cells but is dininished on the fully mature plasma cells. CD22 is an adhesion molecule and plays a role in B cell activation as a signaling molecule.*CD22 antibody has been used to treat leukemia and lymphoma.

Clone: 4AHIB22

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Procedure:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22 (CD22 Products)

Synonyms: A530093D23 antibody, Lyb-8 antibody, Lyb8 antibody, SIGLEC-2 antibody, SIGLEC2 antibody, siglec-2 antibody, CD22 antigen antibody, CD22 molecule antibody, Cd22 antibody, CD22 antibody