p21 antibody (AA 58-77)
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- Target See all p21 (CDKN1A) Antibodies
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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Binding Specificity
- AA 58-77
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This p21 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Recognizes an epitope formed by amino acid residues 58-77 of human p21Cip1/WAF1. Species cross-reactivity: Does not react with the corresponding mouse or rat proteins.
- Purification
- Protein A purified
- Immunogen
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Full length recombinant human p21Cip1/WAF1.
Type of Immunogen: Recombinant protein - Isotype
- IgG1 kappa
- Top Product
- Discover our top product CDKN1A Primary Antibody
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- Application Notes
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Approved: Flo (2 μg/mL), IF (1 - 5 μg/mL), IHC, IHC-Fr, IHC-P, IP, WB
Usage: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-5 μg/mL. Flow Cytometry: 2 μg/mL. Western Blot (chemiluminescence): 0.1-1 μg/mL. Immunohistochemistry (frozen/paraffin): 5 μg/mL. No pretreatment usually needed but some tissues may stain better after pretreating with heat or pressure cooker. Note: In frozen or paraffin sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21 while the proliferating compartment of crypts will not stain. Immunoprecipitation (non-denatured): 2 μg/IP reaction. Immunoprecipitates p21 but not associated proteins. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied s a liquid in PBS, pH 7.4, 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
- Alternative Name
- CDKN1A / WAF1 / p21 (CDKN1A Products)
- Synonyms
- CCND1 antibody, CDKN1A antibody, CAP20 antibody, CDKN1 antibody, CIP1 antibody, MDA-6 antibody, P21 antibody, SDI1 antibody, WAF1 antibody, p21CIP1 antibody, CDKI antibody, Cdkn1 antibody, Waf1 antibody, mda6 antibody, p21Cip1 antibody, p21WAF antibody, Cip1 antibody, UV96 antibody, p21 antibody, cip1 antibody, p16Xic2 antibody, sdi1 antibody, waf1 antibody, cap20 antibody, cdkn1 antibody, mda-6 antibody, p21cip1 antibody, si:ch1073-48m5.2 antibody, KRAS2 antibody, p21ras antibody, cyclin D1 antibody, cyclin dependent kinase inhibitor 1A antibody, cyclin-dependent kinase inhibitor 1A (P21) antibody, cyclin-dependent kinase inhibitor 1A antibody, cyclin-dependent kinase inhibitor 1A L homeolog antibody, cyclin-dependent kinase inhibitor 1 antibody, KRAS proto-oncogene, GTPase antibody, CCND1 antibody, CDKN1A antibody, Cdkn1a antibody, cdkn1a.L antibody, cki1 antibody, cdkn1a antibody, KRAS antibody
- Background
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Name/Gene ID: CDKN1A
Synonyms: CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21 - Gene ID
- 1026
- UniProt
- P38936
- Pathways
- p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
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