CDK4 antibody (AA 1-20)

Catalog No. ABIN227544

Product Details anti-CDK4 Antibody

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Binding Specificity: AA 1-20

Reactivity: Human, Mouse, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Functional Studies (Func)

Specificity: Recognizes p34 (34kD). Does not cross-react with other cdks. Species cross-reactivity: Human, mouse, rat and porcine.

Purification: Protein G purified

Immunogen: Purified recombinant cdk4 protein. Cellular Localization: Nuclear and cytoplasmic. Epitope: aa1-20.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Func, IF, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Kinase assays. Immunoprecipitation Native only, coprecipitates cyclin D's: 2 μg/mg protein lysate. Use protein G. Western Blot: 1-2 μg/mL for 2 hours at RT. Positive control: MAD109, NIH3T3, LS174T, and MCF-7 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4 (CDK4 Products)

Synonyms: zgc:153726 antibody, CMM3 antibody, PSK-J3 antibody, Crk3 antibody, 8-6 antibody, CDK4 antibody, CDK4/6 antibody, CG5072 antibody, Cdk4/6 antibody, DmCdk4 antibody, Dmel\\CG5072 antibody, Pk53C antibody, Pk?7 antibody, cdk antibody, cdk4 antibody, cdk4/6 antibody, dCdk4 antibody, l(2)05428 antibody, l(2)0671 antibody, l(2)k06503 antibody, l(2)s4639 antibody, l(2)sh0671 antibody, xcdk4 antibody, cyclin-dependent kinase 4 antibody, cyclin dependent kinase 4 antibody, Cyclin-dependent kinase 4 antibody, cyclin-dependent kinase 4 L homeolog antibody, cdk4 antibody, CDK4 antibody, Cdk4 antibody, cdk4.L antibody

Background: Name/Gene ID: CDK4
Subfamily: CDC2/CDK
Family: Protein Kinase

Synonyms: CDK4, Cell division protein kinase 4, Crk3, Cyclin-dependent kinase 4, CMM3, p34/cdk4, PSK-J3

Gene ID: 1019

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size