HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Details for Product No. ABIN238485, Supplier: Log in to see
Antigen
  • MHC-G
  • B-F
  • B-F-S04
  • B-F-S05
  • B-F-S06
  • B-F-S07
  • B-FI
  • B-FIV
  • BF2
  • BFa2
  • BFw-03
  • BFw-05
  • BFz-01
  • major histocompatibility complex, class I, G
  • MHC BF1 class I
  • HLA-G
  • BF1
Alternatives
anti-Human HLA Class I Histocompatibility Antigen, alpha Chain G antibody for Blocking Antibody
Reactivity
Human
508
41
9
9
1
1
1
1
Host
Mouse
413
72
26
6
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
52
52
37
27
12
9
9
9
9
9
9
9
9
9
9
8
8
8
7
5
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
301
296
281
156
134
130
118
113
80
23
22
14
8
8
5
3
3
2
2
1
1
Options
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Immunogen Bacterially expressed recombinant HLA-G heavy chain (denaturated).
Clone MEM-G-2
Isotype IgG1
Specificity The antibody MEM-G/2 recognizes the free heavy chain of all the HLA-G isoforms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
Characteristics Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen
Purification Protein A affinity chromatography
Alternative Name HLA Class I alpha G / HLA-G (HLAG Antibody Abstract)
Background HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G
Gene ID 3135
UniProt P17693
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Application Notes Immunohistochemistry (paraffin sections). Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 with 15 mM sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody (ABIN238485) anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody
Background publications Lazana, Zoudiari, Kokkinou, Themeli, Liga, Papadaki, Papachristou, Spyridonidis: "Identification of a novel HLA-G+ regulatory population in blood: expansion after allogeneic transplantation and de novo HLA-G expression at graft-versus-host disease sites." in: Haematologica, Vol. 97, Issue 9, pp. 1338-47, 2012 (PubMed).

Menier, Saez, Horejsi, Martinozzi, Krawice-Radanne, Bruel, Le Danff, Reboul, Hilgert, Rabreau, Larrad, Pla, Carosella, Rouas-Freiss: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." in: Human immunology, Vol. 64, Issue 3, pp. 315-26, 2003 (PubMed).

Créput, Durrbach, Menier, Guettier, Samuel, Dausset, Charpentier, Carosella, Rouas-Freiss: "Human leukocyte antigen-G (HLA-G) expression in biliary epithelial cells is associated with allograft acceptance in liver-kidney transplantation." in: Journal of hepatology, Vol. 39, Issue 4, pp. 587-94, 2003 (PubMed).

Le Discorde, Moreau, Sabatier, Legeais, Carosella: "Expression of HLA-G in human cornea, an immune-privileged tissue." in: Human immunology, Vol. 64, Issue 11, pp. 1039-44, 2003 (PubMed).

Poláková, Bandzuchová, Hofmeister, Weiss, Hutter, Russ: "Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients." in: Neoplasma, Vol. 50, Issue 5, pp. 331-8, 2003 (PubMed).

Boyson, Erskine, Whitman, Chiu, Lau, Koopman, Valter, Angelisova, Horejsi, Strominger: "Disulfide bond-mediated dimerization of HLA-G on the cell surface." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 16180-5, 2002 (PubMed).