This antibody is expected to recognize human HSD11B1 protein. Both NP_005516.1 and NP_861420.1 variants encode the same protein.
Purification
HSD11B1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
HSD11B1 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of HSD11B1.
HSD11B1
Reactivity: Human
ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approximately 35 kDa band observed in human liver lysates (calculated MW of 32.4 kDa according to NP_005516.1 and NP_861420.1). Recommended concentration: 0.3-2 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.