Retinoblastoma 1 antibody (Middle Region)

Catalog No. ABIN2499929

Product Details anti-Retinoblastoma 1 Antibody

Target: Retinoblastoma 1 (RB1)

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TETQAASAFH TQKPLKSTSL ALFYKKVYRL AYLRLNTLCA RLLSDHPELE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Rb1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of MOUSE Rb1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb1 (RB1 Products)

Synonyms: OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, Rb antibody, Rb-1 antibody, P105-RB antibody, PP110 antibody, RB11 antibody, zgc:154147 antibody, RB transcriptional corepressor 1 antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma 1 antibody, RB1 antibody, RBL1 antibody, Rb1 antibody, rb1 antibody

Background: SLC11A1 is key regulator of entry into cell division that acts as a tumor suppressor. It promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. It acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. It controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex By similarity.
Alias Symbols: Rb, Rb-1, pRb
Protein Interaction Partner: Prkcb, Pax2, Prmt2, CDK4, CDK2, Mrfap1, Morf4l1, Jarid2, Cebpd, Cebpb, Cebpa, Neurod1, Cdk6, Rbak, PPP1CA, Cdk9, Ccnd1, Psmd10, Ppp1cc, CSNK2A1, Cdkn1b, E2f1, Suv420h2, Suv420h1, Hdac1, Ubtf, Hmga2, Id2, Hmga1, Runx2, E2f2, ZFPM1, GATA1, TP53BP1, Smarca4,
Protein Size: 921

Molecular Weight: 101 kDa

Gene ID: 19645

NCBI Accession: NM_009029, NP_033055

UniProt: P13405

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy