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HIF1A antibody (Alexa Fluor 647)
HIF1A
Reactivity: Human
IHC, FACS, Func
Host: Mouse
Monoclonal
546-16
Alexa Fluor 647
Product Details anti-HIF1A Antibody (Alexa Fluor 647)
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Target
See all HIF1A Antibodies
HIF1A
(Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
Reactivity
All reactivities for HIF1A antibodies
Human
Host
All hosts for HIF1A antibodies
Mouse
Clonality
All clonalities for HIF1A antibodies
Monoclonal
Conjugate
All conjugates for HIF1A antibodies
This HIF1A antibody is conjugated to Alexa Fluor 647
Application
All applications for HIF1A antibodies
Immunohistochemistry (IHC), Flow Cytometry (FACS), Functional Studies (Func)
Purification
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Clone
546-16
Isotype
IgG2b kappa
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Discover our top product HIF1A Primary Antibody
Alternatives
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anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 341-450) antibody
HIF1A
Reactivity: Human, Mouse, Rat, Rabbit
WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Handling
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Buffer
Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Protect from prolonged exposure to light. Do not freeze.
Storage
4 °C
Storage Comment
The antibody solution should be stored undiluted between 2°C and 8°C
Target Details for HIF1A
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Target
HIF1A
(Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
Alternative Name
HIF1alpha (HIF1A Products )
Synonyms
hif1a antibody, AA959795 antibody, HIF1alpha antibody, MOP1 antibody, bHLHe78 antibody, HIF-1A antibody, HIF-1alpha antibody, HIF1 antibody, HIF1-ALPHA antibody, PASD8 antibody, HIF1-alpha antibody, hif-1a antibody, hypoxia inducible factor 1 alpha antibody, hypoxia inducible factor 1, alpha subunit antibody, hypoxia inducible factor 1 alpha subunit antibody, HIF (Hypoxia Inducible Factor) homologa antibody, hif-1a antibody, Hif1a antibody, HIF1A antibody, hif1a antibody, hif-1 antibody
Background
HIF1, hypoxia induced factor 1, is a heterodimeric basic helix-loop-helix transcription factor composed of HIF1α and HIF1β. HIF1β is an aryl hydrocarbon receptor nuclear translocator (Arnt). Under normoxic conditions, HIF1α undergoes prolyl hydroxylation, which leads to degradation of HIF1α by the proteasome. Oxygen-starvation inhibits prolyl hydroxylation and prevents proteasomal degradation of HIF1α. Following nuclear translocation, HIF1α binds to the core DNA sequence 5'-[AG]CGTG-3' within the hypoxia responsive element (HRE), only the HIF1 heterodimer can bind DNA. HIF1 is a master regulator of the response to hypoxia and induces transcription of multiple genes including VEGF, glucose transporters, and proteins that aid in adaptation to hypoxic conditions. HIF1 protein is also involved in angiogenesis and energy metabolism.
Pathways
Positive Regulation of Peptide Hormone Secretion , Regulation of Hormone Metabolic Process , Regulation of Hormone Biosynthetic Process , Cellular Response to Molecule of Bacterial Origin , Carbohydrate Homeostasis , Transition Metal Ion Homeostasis , Tube Formation , Regulation of Carbohydrate Metabolic Process , Signaling Events mediated by VEGFR1 and VEGFR2 , VEGFR1 Specific Signals , Warburg Effect
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