Core-binding Factor, Runt Domain, alpha Subunit 2, Translocated To, 2 (CBFA2T2) (N-Term) antibody

Details for Product No. ABIN2780644, Supplier: Log in to see
Antigen
  • MTGR1
  • A430091M07
  • C330013D05Rik
  • Cbfa2t2h
  • EHT
  • ZMYND3
  • p85
  • XETOR
  • cbfa2t2-a
  • core-binding factor, runt domain, alpha subunit 2; translocated to, 2
  • core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (human)
  • Cbfa2t2
  • CBFA2T2
  • cbfa2t2
Epitope
N-Term
15
12
6
4
3
3
3
2
2
2
2
1
1
1
1
Reactivity
Human
78
33
27
4
4
4
4
4
3
2
2
1
Host
Rabbit
62
17
5
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
70
26
14
13
9
8
7
7
3
3
1
1
1
1
1
Options
Supplier
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CBFA2T2
Blocking Peptide Blocking peptide for this product available: ABIN974491
Sequence AKESGISLKE IQVLARQWKV GPEKRVPAMP GSPVEVKIQS RSSPPTMPPL
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against CBFA2T2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name CBFA2T2 (CBFA2T2 Antibody Abstract)
Background In acute myeloid leukemia, especially in the M2 subtype, the t(8,21)(q22,q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. CBFA2T2 binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. In acute myeloid leukemia, especially in the M2 subtype, the t(8,21)(q22,q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described.
Alias Symbols: DKFZp313F2116, EHT, MTGR1, ZMYND3, p85
Protein Interaction Partner: PRDM14, PDP1, CBFA2T2, TCP1, MDFI, RUNX1T1, LBP, APP, ATXN1L, ATN1, RERE, RUNX1, SIN3A, NCOR1, HDAC3, FBXL19, TAL2, NEUROG1, ID3, GTF2E1, WDYHV1, CBFA2T3, NCOR2, CBFB, TAL1,
Protein Size: 604
Molecular Weight 67 kDa
Gene ID 9139
NCBI Accession NM_005093, NP_005084
UniProt O43439
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 604 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Core-binding Factor, Runt Domain, alpha Subunit 2, Translocated To, 2 (CBFA2T2) (N-Term) antibody (ABIN2780644) WB Suggested Anti-CBFA2T2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: <...
Western Blotting (WB) image for anti-Core-binding Factor, Runt Domain, alpha Subunit 2, Translocated To, 2 (CBFA2T2) (N-Term) antibody (ABIN2780644) WB Suggested Anti-CBFA2T2
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.1250...
Background publications Kumar, Manning, Spendlove, Kremmidiotis, McKirdy, Lee, Millband, Cheney, Stampfer, Dwivedi, Morris, Callen: "ZNF652, a novel zinc finger protein, interacts with the putative breast tumor suppressor CBFA2T3 to repress transcription." in: Molecular cancer research : MCR, Vol. 4, Issue 9, pp. 655-65, 2006 (PubMed).

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