PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (N-Term)

Details for Product anti-PIAS1 Antibody No. ABIN2780997, Supplier: Log in to see
Antigen
  • 2900068C24Rik
  • Ddxbp1
  • GBP
  • DDXBP1
  • GU/RH-II
  • ZMIZ3
  • ddxbp1
  • gbp
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT 1
  • LOC100232805
  • Pias1
  • PIAS1
  • pias1
Alternatives
anti-Human PIAS1 antibody for Immunofluorescence (fixed cells)
Epitope
N-Term
32
27
18
5
3
3
3
3
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
88
36
19
4
3
3
3
2
2
2
1
1
1
1
Host
Rabbit
83
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Western Blotting (WB)
86
57
52
19
8
6
5
3
2
2
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PIAS1
Blocking Peptide Blocking peptide for this product available: ABIN981725
Sequence MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against PIAS1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background PIAS1 is a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DDXBP1, GBP, GU/RH-II, MGC141878, MGC141879, ZMIZ3
Protein Interaction Partner: STAT1, TRIM63, TRIM55, CEP250, PRDM1, SUMO1, L3MBTL2, CBS, SHFM1, SUMO2, SUMO3, SOX2, CHUK, SPOP, YWHAZ, UBC, SKIL, SGTA, ATXN1, SMAD7, SMAD4, SMAD1, HTT, PRPF40A, PPP1CC, BARD1, PAXIP1, NR1H2, PPARGC1A, NR1H3, PPARGC1B, RAD54L2, ZMYND11, UBD, PLAG1, MYB,
Protein Size: 651
Molecular Weight 72 kDa
Gene ID 8554
NCBI Accession NM_016166, NP_057250
UniProt O75925
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 651 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-PIAS1 antibody (Protein Inhibitor of Activated STAT, 1) (N-Term) (ABIN2780997) anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (N-Term) antibody
Background publications Helbig, Yip, McCartney, Eyre, Beard: "A screening method for identifying disruptions in interferon signaling reveals HCV NS3/4a disrupts Stat-1 phosphorylation." in: Antiviral research, Vol. 77, Issue 3, pp. 169-76, 2008 (PubMed).