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Antibodies
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anti-Glutamate Receptor, Metabotropic 5 (GRM5) antibody
Glutamate Receptor, Metabotropic 5 (GRM5) antibody
| Antigen |
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| Synonyms |
mGlu5, GPRC1E, MGLUR5 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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| Catalog no. |
ABIN302583 |
| Quantity |
50 µg (1.0 mg/ml) (Variants) |
| Price |
390.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Metabotropic glutamate receptor 5, GPRC1E, MGLUR5
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Alternative name
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MGluR5 / GRM5
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Swiss-Prot
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P41594
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Immunogen
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Synthetic peptide - KLH conjugated, N-terminal extracellular domain of humanRemarks: Mouse Identity: 100 %Mouse Similarity: 100 %Rat Identity: 100 %Rat Similarity: 100 %
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Cross-Reactivity
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Human, Mouse (Murine), Rat (Rattus)
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Description
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Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: Ionotropic receptors, and metabotropic receptors(mGluRs). Ionotropic glutamate receptors are oligomeric complexes of various subunits(GluR1 7, NMDA1 3, KAI 2) which comprise ligand gated calcium channels. Metabotropicglutamate receptors are G protein coupled receptors that contain 7 membrane spanningdomains and a large extracellular N terminal region. When activated, they can activatephopholipases or adenylylcyclase, depending on the neuron. The group I receptors mGluR1and mGluR5 activate phospholipase C. By screening a human brain cDNA library with a ratmGluR1 alpha cDNA under low stringency conditions, Minakami et al. (1993) and Minakamiet al. (1994) isolated human cDNAs encoding mGluR5. Minakami et al. (1993) identified alonger isoform of mGluR5 (mGluR5B).
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Specificity
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This antibody detects Metabotropic Glutamate Receptor 5 (GRM5) at N-terminalextracellular domain. Species: Human, Mouse, Rat. Others not tested.
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Application Notes
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Immunohistochemistry on paraffin sections (5.6 ug/ml). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Aff - Purified
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Buffer
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Phosphate buffered saline containing 0.09% sodium azide
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Storage
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Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "Glutamate Receptor, Metabotropic 5 (GRM5)", type "Antibodies"
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Hosts
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Rabbit (18), Mouse (3)
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Reactivities
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Human (17), Rat (Rattus) (10), Mouse (Murine) (4), Rabbit (3), Dog (Canine) (1)
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Applications
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Western Blotting (WB) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Enzyme Immunoassay (EIA) (6), Immunoprecipitation (IP) (4), Flow Cytometry (FACS) (3), Immunofluorescence (IF) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (IHC) (2), ELISA (1), Immunocytochemistry (ICC) (1)
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Epitopes
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C-Term (2), AA 21-509 (1), C-Term T1003 (1)
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