AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Thr450)

Details for Product anti-AKT1 Antibody No. ABIN319376, Supplier: Log in to see
Antigen
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Alternatives
anti-Rat (Rattus) AKT antibody for Dot Blot
Epitope
Thr450
188
123
85
65
56
39
32
30
28
22
21
21
18
18
17
14
13
12
12
11
11
10
10
9
9
9
8
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7
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1137
637
494
63
58
56
46
35
33
30
18
8
8
5
5
5
3
3
3
2
1
1
1
1
Host
Rabbit
856
323
11
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
48
38
35
35
26
25
12
12
7
7
6
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
965
527
435
220
159
149
116
67
56
33
26
15
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
1
Options
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Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 450 (T-I-TP-P-P).
Specificity This antibody detects endogenous levels of total AKT1 protein.
Purification Immunoaffinity Chromatography.
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals
Application Notes Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/100. Immunofluorescence: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Thr450) (ABIN319376) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Thr450) antibody
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Thr450) (ABIN319376) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Thr450) antibody (Image 2)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (Thr450) (ABIN319376) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Thr450) antibody (Image 3)
Background publications Sarbassov, Guertin, Ali, Sabatini: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).

Levin: "It's prime time for reverse transcriptase." in: Cell, Vol. 88, Issue 1, pp. 5-8, 1997 (PubMed).

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