This antibody detects AKT1/PKB pTyr326. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification
Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y326 of human AKT1.
AKT1
Reactivity: Human
WB, IHC, IF, IP
Host: Rabbit
Monoclonal
unconjugated
Application Notes
ELISA: 1/1,000. Western Blot: 1/100-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Dephoure, Zhou, Villén, Beausoleil, Bakalarski, Elledge, Gygi: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, (2008) (PubMed).
AKT1belongs to the protein kinase C superfamily. This kinase is activated bythe Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase