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AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pTyr326)

Details for Product anti-AKT1 Antibody No. ABIN358076, Supplier: Log in to see
Antigen
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Alternatives
anti-Human AKT antibody for Western Blotting
Epitope
pTyr326
188
136
90
87
54
38
37
37
26
25
21
21
21
19
16
15
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15
14
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11
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4
4
4
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3
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3
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2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1162
543
411
44
41
37
34
32
20
18
11
6
5
4
2
2
2
1
1
Host
Rabbit
893
312
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
45
39
35
34
26
25
10
10
5
5
5
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4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
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1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
975
554
446
223
143
120
117
71
63
35
25
11
11
5
3
3
3
3
2
1
1
1
1
1
1
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y326 of human AKT1.
Isotype Ig
Specificity This antibody detects AKT1/PKB pTyr326. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background AKT1belongs to the protein kinase C superfamily. This kinase is activated bythe Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Molecular Weight 55686 Da
Gene ID 207, 9606
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pTyr326) (ABIN358076) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pTyr326) antibody
Product cited in: Dephoure, Zhou, Villén, Beausoleil, Bakalarski, Elledge, Gygi: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).

Background publications McCulloch, Omens: "Myocyte shearing, myocardial sheets, and microtubules." in: Circulation research, Vol. 98, Issue 1, pp. 1-3, 2006 (PubMed).

Margolskee: "Molecular mechanisms of bitter and sweet taste transduction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 1-4, 2002 (PubMed).

Bellacosa, Chan, Ahmed, Datta, Malstrom, Stokoe, McCormick, Feng, Tsichlis: "Akt activation by growth factors is a multiple-step process: the role of the PH domain." in: Oncogene, Vol. 17, Issue 3, pp. 313-25, 1998 (PubMed).

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