Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pTyr701) antibody

Details for Product No. ABIN361957
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Synonyms IMUP, H2RSP, IMUP-1, IMUP-2, 2010005J02Rik, AA408197, DD6G4-4, CANDF7, ISGF-3, STAT91, STAT4, XStat1, STAT, STAT1, dZ182H3.3, si:by134g18.2, si:by51f19.1, si:dz182h3.3, si:dz199m19.1, si:dz73o4.2
(62), (61), (54), (16), (15), (12), (10), (9), (8), (5), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(392), (174), (148), (7), (5), (5), (4), (3), (2), (1), (1)
(334), (82), (5), (5), (4)
(11), (9), (9), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Western Blotting (WB), Immunohistochemistry (IHC)
(384), (152), (140), (82), (72), (44), (30), (26), (20), (11), (10), (9), (9), (7), (4), (4), (1), (1)
Pubmed 6 references available
Quantity 50 μL
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Catalog No. ABIN361957
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Immunogen Peptide sequence around phosphorylation site of pTyr701 (T-G-Y (p) -I-K) derived from Human STAT1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name STAT1
Background Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
Molecular Weight 91 kDa
UniProt P42224
Research Area Cancer, Transcription Factors, Signaling, Receptors, Inflammation
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Demoulin, Van Roost, Stevens et al.: "Distinct roles for STAT1, STAT3, and STAT5 in differentiation gene induction and apoptosis inhibition by interleukin-9." in: The Journal of biological chemistry, Vol. 274, Issue 36, pp. 25855-61, 1999 (PubMed).

Heim: "The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus." in: Journal of receptor and signal transduction research, Vol. 19, Issue 1-4, pp. 75-120, 1999 (PubMed).

Durbin, Hackenmiller, Simon et al.: "Targeted disruption of the mouse Stat1 gene results in compromised innate immunity to viral disease." in: Cell, Vol. 84, Issue 3, pp. 443-50, 1996 (PubMed).

Meraz, White, Sheehan et al.: "Targeted disruption of the Stat1 gene in mice reveals unexpected physiologic specificity in the JAK-STAT signaling pathway." in: Cell, Vol. 84, Issue 3, pp. 431-42, 1996 (PubMed).

Ihle, Witthuhn, Quelle et al.: "Signaling by the cytokine receptor superfamily: JAKs and STATs." in: Trends in biochemical sciences, Vol. 19, Issue 5, pp. 222-7, 1994 (PubMed).

Wakao, Gouilleux, Groner: "Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response." in: The EMBO journal, Vol. 13, Issue 9, pp. 2182-91, 1994 (PubMed).

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