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FAK antibody (PTK2 Protein tyrosine Kinase 2) (pTyr925)

Details for Product anti-PTK2 Antibody No. ABIN362102, Supplier: Log in to see
Antigen
  • FADK
  • FAK
  • FAK1
  • FRNK
  • PPP1R71
  • p125FAK
  • pp125FAK
  • Fadk
  • mKIAA4203
  • PTK2 protein tyrosine kinase 2
  • protein tyrosine kinase 2
  • PTK2
  • Ptk2
  • ptk2
Epitope
pTyr925
73
63
60
46
44
30
25
22
21
16
16
15
15
14
12
10
9
8
8
7
7
5
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
646
449
416
37
23
20
10
10
9
9
8
8
6
6
4
3
3
3
2
2
1
1
Host
Rabbit
566
105
2
2
1
Clonality
Polyclonal
Conjugate
This FAK antibody is un-conjugated
17
16
15
12
12
11
11
11
11
11
11
11
10
10
3
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
457
178
147
142
115
104
58
27
14
7
6
5
5
3
2
Options
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Immunogen Peptide sequence around phosphorylation site of pTyr925 (K-V-Y (p) -E-N) derived from Human FAK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 925.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name FAK (PTK2 Antibody Abstract)
Background Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Molecular Weight 125 kDa
NCBI Accession NP_005598
UniProt Q05397
Research Area Cancer, Extracellular Matrix, Tyrosine Kinases, Angiogenesis, Kinases/Phosphatases
Pathways Response to Growth Hormone Stimulus
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Zheng, Wei, Wang, Gong, Zhang: "Surface characterization and cytocompatibility of three chitosan/polycation composite membranes for guided bone regeneration." in: Journal of biomaterials applications, Vol. 24, Issue 3, pp. 209-29, 2009 (PubMed).

Background publications Sanders, Basson: "p130cas but not paxillin is essential for Caco-2 intestinal epithelial cell spreading and migration on collagen IV." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23516-22, 2005 (PubMed).

Cherubini, Hofmann, Pillozzi, Guasti, Crociani, Cilia, Di Stefano, Degani, Balzi, Olivotto, Wanke, Becchetti, Defilippi, Wymore, Arcangeli: "Human ether-a-go-go-related gene 1 channels are physically linked to beta1 integrins and modulate adhesion-dependent signaling." in: Molecular biology of the cell, Vol. 16, Issue 6, pp. 2972-83, 2005 (PubMed).

Toriumi, Hiraoka, Watanabe, Yoshida: "Pioglitazone reduces monocyte adhesion to vascular endothelium under flow by modulating RhoA GTPase and focal adhesion kinase." in: FEBS letters, Vol. 553, Issue 3, pp. 419-22, 2003 (PubMed).