You are viewing an incomplete version of our website. Please click to reload the website as full version.

FAK antibody (PTK2 Protein tyrosine Kinase 2) (pTyr925)

Details for Product anti-PTK2 Antibody No. ABIN362102, Supplier: Login to see
Antigen
  • FADK
  • FAK
  • FAK1
  • FRNK
  • PPP1R71
  • p125FAK
  • pp125FAK
  • Fadk
  • mKIAA4203
Epitope
pTyr925
69
61
58
50
45
32
22
22
20
15
14
14
13
13
13
13
11
9
9
8
7
7
7
7
6
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
638
357
330
21
6
5
3
3
2
2
2
1
1
1
Host
Rabbit
579
92
2
2
1
Clonality
Polyclonal
Conjugate
This FAK antibody is un-conjugated
18
17
16
13
12
12
11
11
11
11
11
11
3
2
2
1
Application
Immunofluorescence (IF), Western Blotting (WB)
467
182
155
124
102
84
54
24
15
7
6
5
4
3
2
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Immunogen Peptide sequence around phosphorylation site of pTyr925 (K-V-Y (p) -E-N) derived from Human FAK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 925.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name FAK (PTK2 Antibody Abstract)
Background Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Molecular Weight 125 kDa
NCBI Accession NP_005598
UniProt Q05397
Research Area Cancer, Extracellular Matrix, Tyrosine Kinases, Angiogenesis, Kinases/Phosphatases
Pathways
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Zheng, Wei, Wang et al.: "Surface characterization and cytocompatibility of three chitosan/polycation composite membranes for guided bone regeneration." in: Journal of biomaterials applications, Vol. 24, Issue 3, pp. 209-29, 2009 (PubMed).

Background publications Sanders, Basson: "p130cas but not paxillin is essential for Caco-2 intestinal epithelial cell spreading and migration on collagen IV." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23516-22, 2005 (PubMed).

Cherubini, Hofmann, Pillozzi et al.: "Human ether-a-go-go-related gene 1 channels are physically linked to beta1 integrins and modulate adhesion-dependent signaling." in: Molecular biology of the cell, Vol. 16, Issue 6, pp. 2972-83, 2005 (PubMed).

Toriumi, Hiraoka, Watanabe et al.: "Pioglitazone reduces monocyte adhesion to vascular endothelium under flow by modulating RhoA GTPase and focal adhesion kinase." in: FEBS letters, Vol. 553, Issue 3, pp. 419-22, 2003 (PubMed).