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Retinoblastoma 1 antibody (RB1) (pSer780)

Details for Product anti-RB1 Antibody No. ABIN362117, Supplier: Log in to see
Antigen
Alternatives
anti-Human Retinoblastoma 1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
pSer780
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This Retinoblastoma 1 antibody is un-conjugated
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Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
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Supplier
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Immunogen Peptide sequence around phosphorylation site of pSer780 (T-L-S (p) -P-I) derived from Human Rb. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of Rb only when phosphorylated at serine 780.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Rb (RB1 Antibody Abstract)
Background Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Molecular Weight 110 kDa
NCBI Accession NP_000312
UniProt P06400
Research Area Cancer, Transcription Factors
Pathways Cell Division Cycle
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Cai, Ye, Liu et al.: "Luteolin induced G2 phase cell cycle arrest and apoptosis on non-small cell lung cancer cells." in: Toxicology in vitro : an international journal published in association with BIBRA, Vol. 25, Issue 7, pp. 1385-91, 2011 (PubMed).

Background publications Roesch, Becker, Meyer et al.: "Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 4, pp. 565-72, 2005 (PubMed).

Huang, Zegarra-Moro, Benson et al.: "Androgens repress Bcl-2 expression via activation of the retinoblastoma (RB) protein in prostate cancer cells." in: Oncogene, Vol. 23, Issue 12, pp. 2161-76, 2004 (PubMed).

Kitagawa, Higashi, Jung et al.: "The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2." in: The EMBO journal, Vol. 15, Issue 24, pp. 7060-9, 1997 (PubMed).