c-MYC antibody (AA 356-360)

Catalog No. ABIN362549

There are 2+ publications for this product available. The Rabbit Polyclonal anti-c-MYC antibody is suitable to detect c-MYC in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for c-MYC antibody (AA 356-360) (ABIN362549)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-c-MYC Antibody

Binding Specificity: AA 356-360

Specificity: The antibody detects endogenous level of total Myc protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 356-360 (R-R-T-H-N) derived from Human Myc. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-c-MYC antibody (ABIN362549)

Giugliano, Ruff, Braunwald, Murphy, Wiviott, Halperin, Waldo, Ezekowitz, Weitz, Špinar, Ruzyllo, Ruda, Koretsune, Betcher, Shi, Grip, Patel, Patel, Hanyok, Mercuri, Antman: "Edoxaban versus warfarin in patients with atrial fibrillation." in: The New England journal of medicine, Vol. 369, Issue 22, pp. 2093-104, (2013) (PubMed).

Liu, Ma, Lee: "NMDA receptor signaling mediates the expression of protein inhibitor of activated STAT1 (PIAS1) in rat hippocampus." in: Neuropharmacology, Vol. 65, pp. 101-13, (2012) (PubMed).

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: Myc Proto-Oncogene protein

Background: Myc proto-oncogene encodes nuclear DNA-binding phosphoproteins that are involved in the regulation of gene expression and DNA replication during cell growth and differentiation. Myc encodes a protein of 65 kDa which is expressed in almost all normal and transformed cells. The expression correlates with the proliferation state of the cells. Transcription is repressed in quiescent or terminally differentiated cells. Expression of Myc is generally induced after mitogenic stimulation of cells or serum induction. Myc therefore is an important positive regulator of cell growth and proliferation. Myc has been demonstrated also to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. Apoptosis may serve also as a protective mechanism to prevent tumorigenicity elicited by deregulated Myc expression. Sequences of the Myc oncogene have been highly conserved throughout evolution, from Drosophila to vertebrates

Molecular Weight: 60 kDa

NCBI Accession: NP_002458

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect