Nuclear Factor kappa B p50 (NFkB p50) (AA 925-929) antibody

Details for Product No. ABIN363027, Supplier: Log in to see
Antigen
Epitope
AA 925-929
22
22
15
13
12
12
12
6
5
5
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
216
104
75
10
9
9
5
1
Host
Rabbit
183
34
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
181
103
59
43
39
20
13
11
7
6
5
2
2
2
1
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Immunogen Peptide sequence around AA 925-929 (C-D-S-G-V ) derived from Human NFkB-p105. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total NFkB-p105 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name NFkappaB-p105/p50
Background NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.
Molecular Weight 120 kDa
NCBI Accession NP_001158
UniProt P19838
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Hou, Guan, Ricciardi: "Phosphorylation of serine 337 of NF-kappaB p50 is critical for DNA binding." in: The Journal of biological chemistry, Vol. 278, Issue 46, pp. 45994-8, 2003 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Haskill, Beg, Tompkins, Morris, Yurochko, Sampson-Johannes, Mondal, Ralph, Baldwin: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

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