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STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (C-Term)

Details for Product anti-STAT3 Antibody No. ABIN374087, Supplier: Log in to see
Antigen
  • 1110034C02Rik
  • APRF
  • Aprf
  • aprf
  • AW109958
  • hies
  • HIES
  • stat3
  • wu:fc15d02
  • wu:fl59g06
  • Xstat3
  • z-Stat3
Alternatives
anti-Human STAT3 antibody for Western Blotting
Epitope
C-Term, Isoform 1
111
91
84
27
20
17
17
16
16
14
13
13
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
550
274
214
29
23
23
17
10
5
5
4
3
3
2
2
2
2
2
1
1
1
1
Host
Goat
456
117
34
5
1
1
Clonality
Polyclonal
Conjugate
This STAT3 antibody is un-conjugated
24
22
18
13
11
11
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
503
245
223
84
74
72
57
37
23
12
11
11
8
7
3
3
3
2
2
2
1
1
1
1
Supplier
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Immunogen Peptide from C-Terminus of the protein sequence according to NP_003141.2, NP_644805.1. Genename: STAT3
Sequence DMELTSECAT SPM
Specificity This antibody is expected to recognize Isoform 1 (NP_644805.1) and Isoform 2 (NP_003141.2).
Cross-Reactivity (Details) Species reactivity (expected):Canine (Dog), Pig and Bovine.
Species reactivity (tested):Human, Mouse, Rat.
Purification Immunoaffinity Chromatography
Alternative Name STAT3 (STAT3 Antibody Abstract)
Background The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3
Gene ID 6774
NCBI Accession NP_003141
UniProt P40763
Research Area Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway
Application Notes Peptide ELISA: 1/128000. Western blot: 1-3 μg/mL. Approx.90 kDa band observed in lysates of NIH3T3 (calculatedMW of 88.0 kDa according to NP_644805.1 and of 88.1 according to NP_003141.2). Immunohistochemistry: Rommended overnight staining at 10 μg/mL (See picture). Stainsmouse thymic medulla and not the cortex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-STAT3 antibody (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor)) (C-Term) (ABIN374087) anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term), (Isoform 1) antibody
Background publications Bromberg, Wrzeszczynska, Devgan, Zhao, Pestell, Albanese, Darnell: "Stat3 as an oncogene." in: Cell, Vol. 98, Issue 3, pp. 295-303, 1999 (PubMed).