C-Myc-Tag antibody (HRP)

Antigen: C-Myc-Tag

Clonality: Monoclonal (2G8D5)

Host: Mouse

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Certificates: ISO 9001:2008

Catalog no.: ABIN387706

Additional Information

Immunogen: A synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH

Format: Purified

Isotype: IgG2a

Clone: 2G8D5

Description: Antibodies-Online Anti-c-Myc [HRP] Monoclonal Antibody is immunoaffinity purified from mice ascites with protein A and then conjugated to horseradish peroxidase. Mouse Anti-c-Myc [HRP] Monoclonal Antibody is suitable for detecting the expression level of c-Myc in fusion proteins.

Specificity: Antibodies-Online Anti-c-Myc [HRP] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal c-Myc-tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes the c-Myc gene in human cancers, such as Hela whole cell lysate.

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by severalfactors, including secondary antibody affinity, antigen concentration,sensitivity of detection method, temperature, and length of incubations,etc. The suitability of this antibody for applications other than thoselisted below has not been determined. The following concentration rangesare recommended starting points for this product.ELISA: 0.05?.2 ug/ml Western blot: 0.1-1 ug/mlImmunoprecipitation (IP): 1 ug/mlImmunofluorescent staining: 1 ug/ml Other applications: user-optimized

Concentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal

Purification: Protein A affinity column

Storage: GenScript Anti-c-Myc [HRP] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at or below -20°C. Avoid repeated freezing and thawing cycles.

Restrictions: For Research Use only

Images

Lane 1 and 2: Multiple Tag Cell Lysate (Antibodies-Online, M0100)Primary antibody: 0.1 µg/ml Anti-c-Myc [HRP] Monoclonal Antibody (Mouse) (Antibodies-Online, ABIN396862)The signal was developed with LumiSensor HRP Substrate Kit (Antibodies-Online, L00221V500)