C-Myc-Tag antibody (Biotin)

Antigen: C-Myc-Tag

Clonality: Monoclonal (2G8D5)

Host: Mouse

Conjugate: Biotin

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN387707

Additional Information

Immunogen: A synthetic peptide EQKLISEEDL coupled to KLH

Format: Purified

Isotype: IgG2a, kappa chain

Clone: 2G8D5

Description: Antibodies-Online Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) is the biotin conjugated Anti-c-Myc Monoclonal Antibody (Mouse) (ABIN396860) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.

Specificity: Antibodies-Online Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc gene in human cancers, such as Hela whole cell lysates.

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/ml Western blot: 0.1-1 ug/ml Other applications: user-optimized

Concentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide

Purification: Protein A affinity column

Storage: Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Restrictions: For Research Use only

Images

Lane 1-3: 200 ng, 50 ng, 10 ng Multiple Tag Cell Lysate (Antibodies-Online, M0100)Lane 4: Negative ControlDetect antibody: 0.1 ug/ml Anti-c-Myc [Biotin] Monoclonal Antibody (Mouse) (Antibodies-Online, ABIN396863)Secondary antibody: Streptavidin-HRP (Antibodies-Online, M00091, 1:10,000)The signal was developed with LumiSensor HRP Substrate Kit (Antibodies-Online, L00221V500)