Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (C-Term), (AA 134-164) antibody

Details for Product No. ABIN391763
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Antigen
Epitope
C-Term, AA 134-164
(43), (40), (24), (23), (13), (12), (11), (9), (9), (7), (6), (6), (6), (6), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(283), (88), (63), (24), (24), (12), (12), (12), (2), (1), (1)
Host
Rabbit
(229), (80), (2), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(15), (15), (15), (9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
(187), (105), (87), (79), (50), (47), (42), (33), (18), (14), (7), (5), (2), (2), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN391763
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Immunogen This p21 (CDKN1A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-164 AA from the C-terminal region of human p21 (CDKN1A).
Clone RB3180
Isotype Ig
Specificity This p21 (CDKN1A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135~164 amino acids from the C-terminal region of human CDKN1A.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name p21 (CDKN1A)
Background CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
Synonyms: Cyclin-dependent kinase inhibitor 1, p21, CDK-interacting protein 1, Melanoma differentiation associated protein 6, MDA-6
Molecular Weight 18119 DA
Gene ID 1026
UniProt P38936
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (C-Term), (AA 134-164) antibody The anti-CDKN1A Pab (ABIN391763) is used in Western blot to detect CDKN1A in T-47D cell lysate.
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (C-Term), (AA 134-164) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Di Padova, Bruno, De Nicola et al.: "Che-1 arrests human colon carcinoma cell proliferation by displacing HDAC1 from the p21WAF1/CIP1 promoter." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36496-504, 2003 (PubMed).

Dupont, Karas, LeRoith: "The cyclin-dependent kinase inhibitor p21CIP/WAF is a positive regulator of insulin-like growth factor I-induced cell proliferation in MCF-7 human breast cancer cells." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37256-64, 2003 (PubMed).

Frouin, Maga, Denegri et al.: "Human proliferating cell nuclear antigen, poly(ADP-ribose) polymerase-1, and p21waf1/cip1. A dynamic exchange of partners." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39265-8, 2003 (PubMed).

Bai, Miyake, Iwai et al.: "CDX2, a homeobox transcription factor, upregulates transcription of the p21/WAF1/CIP1 gene." in: Oncogene, Vol. 22, Issue 39, pp. 7942-9, 2003 (PubMed).

Fukuchi, Nakamura, Ichimura et al.: "The association of cyclin A and cyclin kinase inhibitor p21 in response to gamma-irradiation requires the CDK2 binding region, but not the Cy motif." in: Biochimica et biophysica acta, Vol. 1642, Issue 3, pp. 163-71, 2003 (PubMed).

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