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P21-Activated Kinase 1 (PAK1) (AA 401-430), (Thr423) antibody

Details for Product No. ABIN392432
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Antigen
Epitope
AA 401-430, Thr423
(38), (18), (14), (12), (12), (10), (8), (8), (6), (5), (5), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(228), (128), (127), (38), (12), (1)
Host
Rabbit
(216), (10), (5), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(7), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
(168), (90), (88), (51), (40), (36), (19), (14), (14), (2)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN392432
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Immunogen This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 AA from human PAK1.
Clone RB11382
Isotype Ig
Specificity This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T423 of human PAK1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus),Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name PAK1
Background PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.
Synonyms: Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, P65-PAK, Alpha-PAK
Molecular Weight 60661 DA
Gene ID 5058
UniProt Q13153
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes WB = 1:1000, IF = 1:10-50, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.43 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-P21-Activated Kinase 1 (PAK1) (AA 401-430), (Thr423) antibody Western blot analysis of PAK1 (arrow) using rabbit polyclonal PAK1 Antibody (T423) (ABIN392432).293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2) (Origene Technologies).
anti-P21-Activated Kinase 1 (PAK1) (AA 401-430), (Thr423) antibody (2) Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 Antibody (T423) (ABIN392432), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Chen, Yin, Zhu et al.: "The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20029-36, 2003 (PubMed).

Validation Images
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