V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Thr308) antibody

Details for Product No. ABIN400786
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Antigen
Synonyms
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, pkb, xAct, akt-1, v-akt, v-akt1, Akt, AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AKT/PKB, Akt/PKB, Akt1, CG4006, D-Akt,  ... show more
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, pkb, xAct, akt-1, v-akt, v-akt1, Akt, AKT1, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, AKT/PKB, Akt/PKB, Akt1, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AKT, PKB/Akt, PKB/dAKT, RacPK, akt, akt1, dAKT, dAKT/dPKB, dAKT1, dAkt, dAkt/PKB, dAkt1, dPKB, dakt, dakt1, l(3)04226, l(3)89Bq, p-Akt, pAkt show less
Epitope
Thr308
(129), (88), (54), (50), (48), (33), (32), (29), (28), (17), (16), (16), (10), (9), (9), (8), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(837), (536), (514), (167), (153), (115), (94), (21), (18), (4), (3), (1), (1), (1), (1)
Host
Mouse
(699), (143), (7), (5), (4), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(20), (14), (13), (13), (13), (13), (13), (13), (13), (13), (13), (9), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(637), (360), (240), (160), (139), (130), (123), (65), (43), (17), (11), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN400786
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
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Immunogen Synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein
Clone 18F3-H11
Isotype IgG1
Characteristics Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase
Purification Affinity chromatography on Protein A
Alternative Name AKT1 / PKB
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB)and rac (related to A and C kinases). AKT is a key regulator of many signal transductionpathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKTmediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growthfactors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it isactivated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from themembrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has twomain roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Application Notes ELISA: 1/20,000. Western Blot: 1/500 - 1/3,000. Immunohistochemistry: 20 µg/ml. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1,48 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative
Preservative Sodium azide
Storage -20 °C
Storage Comment Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Thr308) antibody anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Thr308) antibody
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Thr308) antibody (2) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Thr308) antibody (Image 2)
Background publications Jones, Jakubowicz, Pitossi et al.: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991 (PubMed).

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987 (PubMed).

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001 (PubMed).

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001 (PubMed).

Hosts (699), (143), (7), (5), (4), (3)
Reactivities (837), (536), (514), (167), (153), (115), (94), (21), (18), (4), (3), (1), (1), (1), (1)
Applications (637), (360), (240), (160), (139), (130), (123), (65), (43), (17), (11), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Conjugates (20), (14), (13), (13), (13), (13), (13), (13), (13), (13), (13), (9), (7), (7), (7), (7), (1), (1), (1), (1), (1), (1)
Epitopes (129), (88), (54), (50), (48), (33), (32), (29), (28), (17), (16), (16), (10), (9), (9), (8), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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