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Retinoblastoma 1 antibody (RB1) (C-Term)

Details for Product anti-RB1 Antibody No. ABIN453807, Supplier: Log in to see
Antigen
  • RB1
  • OSRC
  • RB
  • p105-Rb
  • pRb
  • pp110
  • Rb
  • Rb-1
  • zgc:154147
  • P105-RB
  • PP110
  • RB11
  • retinoblastoma 1 (including osteosarcoma)
  • retinoblastoma 1
  • Retinoblastoma-associated protein
  • RB1
  • rb
  • Rb1
  • rb1
Alternatives
anti-Human Retinoblastoma 1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
C-Term
52
51
51
41
37
30
28
20
18
18
16
15
15
14
8
8
8
7
7
7
6
6
5
5
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
616
355
251
11
8
7
5
4
4
3
2
2
2
2
Host
Rabbit
462
156
2
Clonality
Polyclonal
Conjugate
This Retinoblastoma 1 antibody is un-conjugated
28
26
25
16
14
14
13
13
7
7
7
7
7
7
7
7
7
5
5
5
5
5
5
5
5
3
2
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
376
248
174
148
94
88
45
38
26
25
10
7
7
5
5
2
2
2
1
1
Options
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Immunogen KLH conjugated synthetic peptide selected from the C-terminal region of human RB1
Specificity This antibody reacts to RB1.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Alternative Name Retinoblastoma-Associated Protein / RB1 (RB1 Antibody Abstract)
Background RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.Synonyms: OSRC, Rb, Retinoblastoma 1, p105-Rb, pRb, pp110
Gene ID 5925
NCBI Accession NP_000312
UniProt P06400
Pathways Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium az
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Retinoblastoma 1 antibody (RB1) (C-Term) (ABIN453807) Western blot analysis of RB1 Antibody
Background publications Connell-Crowley, Harper, Goodrich: "Cyclin D1/Cdk4 regulates retinoblastoma protein-mediated cell cycle arrest by site-specific phosphorylation." in: Molecular biology of the cell, Vol. 8, Issue 2, pp. 287-301, 1997 (PubMed).

Kitagawa, Higashi, Jung, Suzuki-Takahashi, Ikeda, Tamai, Kato, Segawa, Yoshida, Nishimura, Taya: "The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2." in: The EMBO journal, Vol. 15, Issue 24, pp. 7060-9, 1997 (PubMed).