ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (pTyr302)

Details for Product anti-ARAF Antibody No. ABIN482099, Supplier: Log in to see
Antigen
  • zgc:92074
  • wu:fk45h07
  • ARAF
  • a-raf
  • araf
  • araf1
  • pks2
  • rafa1
  • A-RAF
  • ARAF1
  • PKS2
  • RAFA1
  • 1200013E08Rik
  • A-Raf
  • AW495444
  • Araf1
  • v-raf murine sarcoma 3611 viral oncogene homolog
  • araf
  • ARAF
  • LOC100340437
  • araf-a
  • araf-b
  • Araf
Alternatives
anti-Human ARAF antibody for Western Blotting
Epitope
pTyr302
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Rabbit
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26
Clonality
Polyclonal
Conjugate
This ARAF antibody is un-conjugated
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Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302.
Isotype IgG
Specificity A-RAF (Phospho-Tyr302) Antibody detects endogenous levels of A- RAF only when phosphorylated at Tyr302.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Alternative Name A-RAF (ARAF Antibody Abstract)
Background Molecular weight of target protein: 67 kDa
Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, Proto- oncogene Pks, ARAF, ARAF1, PKS, PKS2
Molecular Weight 67 kDa
Gene ID 369
OMIM 311010
UniProt P10398
Pathways MAPK Signaling, RTK Signaling, Hepatitis C
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.225936, Hs.446641 (NCBI Gene Symbol: ARAF)

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pTyr302) antibody (ABIN482099) Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', usin...
Immunohistochemistry (IHC) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pTyr302) antibody (ABIN482099) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RA...
Immunofluorescence (IF) image for anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pTyr302) antibody (ABIN482099) Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. Th...
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