HLA-E antibody (Biotin)
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- Target See all HLA-E Antibodies
- HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HLA-E antibody is conjugated to Biotin
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Specificity
- The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denatured heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
- Purification
- Protein A purified
- Immunogen
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Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Type of Immunogen: Synthetic peptide - Clone
- MEM-E-07
- Isotype
- IgG1
- Top Product
- Discover our top product HLA-E Primary Antibody
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- Application Notes
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Approved: Flo, IF
Usage: Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working dilution is 2 microgram/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determin The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 15 mM sodium azide, pH 7.4
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
- Alternative Name
- HLA-E (HLA-E Products)
- Synonyms
- EA1.2 antibody, EA2.1 antibody, HLA-6.2 antibody, MHC antibody, QA1 antibody, major histocompatibility complex, class I, E antibody, HLA-E antibody, PATR-E antibody
- UniProt
- P13747
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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