STAT5B antibody
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- Target See all STAT5B Antibodies
- STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This STAT5B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of Stat5b protein.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity chromatography using epitope-specific immunogen
- Purity
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product STAT5B Primary Antibody
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- Application Notes
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- Target
- STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))
- Alternative Name
- STAT5B (STAT5B Products)
- Synonyms
- STAT5 antibody, stat5 antibody, STAT5A antibody, STAT5B antibody, signal transducer and activator of transcription 5B antibody, signal transducer and activator of transcription 5B S homeolog antibody, Signal transducer and activator of transcription 5B antibody, STAT5B antibody, Stat5b antibody, stat5b.S antibody, LOC490969 antibody, sta5b antibody, LOC100566752 antibody, LOC100052954 antibody
- Background
- Stat5a and Stat5b (signal transducer and activator of transcription 5a and 5b, 96 % homology) undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7, GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phosphorylation of Stat5a at Ser 127/Ser 128 and Ser 779 are contigent on ErbB4-mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with retinoic acid receptor-α gene in acute promyelocytic leukemias (APLL). Stat5a/b null mice have severely impaired lymphoid development and differentiation.Synonyms: STAT-5B, Signal transducer and activator of transcription 5B
- Molecular Weight
- approx. 90 kDa
- Gene ID
- 6777
- NCBI Accession
- NP_036580
- UniProt
- P51692
- Pathways
- JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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