MDM2 antibody (C-Term)
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- Target See all MDM2 Antibodies
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
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Binding Specificity
- AA 343-358, C-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MDM2 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Purpose
- Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2(MDM2) detection. Tested with WB in Human,Mouse,Rat.
- Sequence
- EKAKLENSTQ AEEGFD
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2(MDM2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: MDM2 oncogene, E3 ubiquitin protein ligase
Protein Name: E3 ubiquitin-protein ligase Mdm2 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2(343-358aa EKAKLENSTQAEEGFD), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product MDM2 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Sodium azide, Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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Fra-1 is downregulated in cervical cancer tissues and promotes cervical cancer cell apoptosis by p53 signaling pathway in vitro." in: International journal of oncology, Vol. 46, Issue 4, pp. 1677-84, (2015) (PubMed).
: "The oncoprotein HBXIP modulates the feedback loop of MDM2/p53 to enhance the growth of breast cancer." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22649-61, (2015) (PubMed).
: "CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer." in: Oncology reports, Vol. 32, Issue 6, pp. 2703-9, (2014) (PubMed).
: "
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Fra-1 is downregulated in cervical cancer tissues and promotes cervical cancer cell apoptosis by p53 signaling pathway in vitro." in: International journal of oncology, Vol. 46, Issue 4, pp. 1677-84, (2015) (PubMed).
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- Target
- MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
- Alternative Name
- MDM2 (MDM2 Products)
- Synonyms
- ACTFS antibody, HDMX antibody, hdm2 antibody, zgc:109834 antibody, 1700007J15Rik antibody, AA415488 antibody, Mdm-2 antibody, MDM2 proto-oncogene antibody, MDM2 proto-oncogene S homeolog antibody, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antibody, MDM2 oncogene, E3 ubiquitin protein ligase antibody, transformed mouse 3T3 cell double minute 2 antibody, MDM2 antibody, mdm2.S antibody, Mdm2 antibody, mdm2 antibody
- Background
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Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.
Synonyms: Double minute 2 protein antibody|E3 ubiquitin-protein ligase Mdm2 antibody|Hdm 2 antibody|HDM2 antibody|MDM 2 antibody|MDM2 antibody|Mdm2 transformed 3T3 cell double minute 2 p53 binding protein(mouse) binding protein 104 kDa antibody|MDM2_HUMAN antibody|MDM2BP antibody|Mouse Double Minute 2 antibody|MTBP antibody|Murine Double Minute Chromosome 2 antibody|Oncoprotein Mdm2 antibody|p53 Binding Protein Mdm2 antibody|p53-binding protein Mdm2 antibody|Ubiquitin protein ligase E3 Mdm2 antibody - UniProt
- Q00987
- Pathways
- p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway
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