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CD22 antibody (CD22 Molecule) (C-Term)

Details for Product anti-CD22 Antibody No. ABIN611290, Supplier: Log in to see
Antigen
  • A530093D23
  • Lyb-8
  • Lyb8
  • SIGLEC-2
  • SIGLEC2
  • siglec-2
  • CD22 antigen
  • CD22 molecule
  • Cd22
  • CD22
Epitope
C-Term
30
21
17
10
10
10
8
6
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Horse (Equine), Human
507
194
29
17
11
6
6
5
3
Host
Rabbit
397
158
107
1
Clonality
Polyclonal
Conjugate
This CD22 antibody is un-conjugated
94
74
42
24
15
13
11
11
11
9
9
9
9
9
8
8
7
7
7
6
4
2
2
2
2
2
2
2
1
1
1
Application
Western Blotting (WB)
511
174
74
72
66
61
54
43
40
9
9
7
4
3
3
3
2
2
2
2
1
1
1
Options
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Immunogen Synthetic peptide from C-Terminus of human CD22 (P20273, NP_001762). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset (92%).

Type of Immunogen: Synthetic peptide
Specificity Human CD22
Predicted Reactivity Percent identity by BLAST analysis: Human (100%).
Purification Immunoaffinity purified
Alternative Name CD22 (CD22 Antibody Abstract)
Background Name/Gene ID: CD22
Family: Immunoglobulin

Synonyms: CD22, CD22 Molecule, CD22 antigen, SIGLEC2, SIGLEC-2, T-cell surface antigen Leu-14, B-cell receptor CD22, BL-CAM
Gene ID 933
NCBI Accession NP_001762
UniProt P20273
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Supplier Images
 image for anti-CD22 antibody (CD22 Molecule) (C-Term) (ABIN611290) anti-CD22 Molecule (CD22) (C-Term) antibody
Product cited in: Waite, Floyd, Arbour-Reily, Stephens: "Interferon-gamma-induced regulation of peroxisome proliferator-activated receptor gamma and STATs in adipocytes." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7062-8, 2001 (PubMed).

Bartoli, Gu, Tsai, Venema, Brooks, Marrero, Caldwell: "Vascular endothelial growth factor activates STAT proteins in aortic endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 43, pp. 33189-92, 2000 (PubMed).

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, 2000 (PubMed).

Dent, Hu-Li, Paul, Staudt: "T helper type 2 inflammatory disease in the absence of interleukin 4 and transcription factor STAT6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13823-8, 1998 (PubMed).

Heim: "The Jak-STAT pathway: specific signal transduction from the cell membrane to the nucleus." in: European journal of clinical investigation, Vol. 26, Issue 1, pp. 1-12, 1996 (PubMed).

Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996 (PubMed).

Hou, Schindler, Henzel, Ho, Brasseur, McKnight: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994 (PubMed).

Background publications Quelle, Shimoda, Thierfelder, Fischer, Kim, Ruben, Cleveland, Pierce, Keegan, Nelms: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995 (PubMed).