WB: 0.2-2 µg/mL, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL Optimal conditions should be determined by the investigator.
Restrictions
For Research Use only
Concentration
Lot specific
Buffer
CBFA2 T2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3).
Preservative
Sodium azide
Precaution of Use
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Storage
4 °C/-20 °C
Storage Comment
Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.
Target
CBFA2T2
(Core-binding Factor, Runt Domain, alpha Subunit 2, Translocated To, 2 (CBFA2T2))
In acute myeloid leukemia, especially in the M2 subtype, the t(8,21)(q22,q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. Synonyms: Monoclonal CBFA2T2 antibody, Anti-CBFA2T2 antibody, Core-binding factor runt domain alpha subunit 2 antibody, MTGR1 antibody, EHT antibody, ZMYND3 antibody.