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Antibodies
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anti-Signal Transducer and Activator of Transcription 1 (STAT1) antibody
Signal Transducer and Activator of Transcription 1 (STAT1) antibody
| Antigen |
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| Synonyms |
ISGF-3, STAT91, DKFZp686B04100, XStat1, STAT1, STAT4, isgf-3, stat91, MGC147452, DKFZp459D206 |
| Clonality |
Monoclonal (SM1) |
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Host
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Reactivity
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Human (104), Mouse (Murine) (49), Rat (Rattus) (46), Dog (Canine) (21), Cow (Bovine) (19), Chicken (16), Horse (Equine) (4), Pig (Porcine) (4), Monkey (3), Rabbit (3), Sheep (Ovine) (1), Zebrafish (Danio rerio) (1)
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Application
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 Alternatives Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (95), Immunofluorescence (IF) (32), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Immunoprecipitation (IP) (23), Flow Cytometry (FACS) (19), Enzyme Immunoassay (EIA) (17), Immunohistochemistry (IHC) (15), ELISA (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Dot Blot (Dot) (3), ELISA (Detection) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN94471 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
325.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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STAT1
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Immunogen
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STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).
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Format
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Purified
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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SM1
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Description
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STAT1 (signal transducer and activator of transcription 1) is a transcription factor that playsimportant roles in growth arrest, apoptosis promoting and tumour suppression. After ligationof cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 orJAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimersserve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is alsophosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation.However, the both modifications are important for its maximal transcriptional activity. On theother hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
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Specificity
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The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a91 kDa transcriptional factor involved in a variety of systems including antiviral responsesand interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
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Application Notes
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Immunohistochemistry (paraffin sections) - Recommended dilution: 5 µg/ml. Positive tissue: lung macrophages
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from hybridoma culture supernatant by protein A-affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Cancer, Transcription Factors, Signaling, Receptors, Inflammation
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Restrictions
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For Research Use only
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Alternatives for antigen "Signal Transducer and Activator of Transcription 1 (STAT1)", type "Antibodies"
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Hosts
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Rabbit (84), Mouse (26), Goat (2), Hamster (2), Chicken (1)
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Reactivities
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Human (104), Mouse (Murine) (49), Rat (Rattus) (46), Dog (Canine) (21), Cow (Bovine) (19), Chicken (16), Horse (Equine) (4), Pig (Porcine) (4), Monkey (3), Rabbit (3), Sheep (Ovine) (1), Zebrafish (Danio rerio) (1)
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Applications
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Western Blotting (WB) (95), Immunofluorescence (IF) (32), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Immunoprecipitation (IP) (23), Flow Cytometry (FACS) (19), Enzyme Immunoassay (EIA) (17), Immunohistochemistry (IHC) (15), ELISA (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Dot Blot (Dot) (3), ELISA (Detection) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pSer727 (11), pTyr701 (9), transcript variant beta (4)
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