Histone H2A.X (Ab-139) antibody detects endogenous levels of total Histone H2A.X protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
H2AFX
Reactivity: Human
WB, IF, ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Yaneva, Li, Marple, Hasty: "Non-homologous end joining, but not homologous recombination, enables survival for cells exposed to a histone deacetylase inhibitor." in: Nucleic acids research, Vol. 33, Issue 16, pp. 5320-30, (2005) (PubMed).