Images for product: anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibody
Western blot analysis of H2AX (pS139) in transformed human epithelioid carcinoma. Lysates from control (first panel) and Staurosporine-treated (second panel) HeLa cells (ATCC CCL-2) were probed with Purified Mouse anti-H2AX (pS139) monoclonal antibody at concentrations of 0.25, 0.125, and 0.06 µg/ml (Lanes 1, 2, and 3, respectively). H2AX (pS139 )is identified as a band of ~15 kDa with increased density in the treated cells.
Immunofluorescent staining of human cell line. HeLa cells (ATCC CCL-2) were seeded in a 96-well Imaging Plate at ~10,000 cells per well. After overnight culture, the cells were exposed to 2400 Joules UV irradiation (right image) or untreated (left image) and then allowed to recover for 30-60 minutes at 37°C. The cells were fixed, permeabilized with cold methanol, and stained with Purified Mouse anti-H2AX (pS139) followed by Alexa Fluor® 488 goat anti-mouse IgG (Invitrogen, pseudo colored green) according to the Recommended Assay Procedure. Cell nuclei were counterstained with Hoechst 33342 (pseudo colored blue). The images were captured on a BD Pathway™ 435 high-content Bioimager system using a 20X objective and merged using BD AttoVision™ software. This antibody also worked with the Saponin and the Triton™ X-100 Perm/Wash protocols (see Recommended Assay Procedure, Bioimaging protocol link).