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ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (AA 183-199)

Details for Product anti-ARAF Antibody No. ABIN967727, Supplier: Log in to see
Antigen
  • zgc:92074
  • wu:fk45h07
  • ARAF
  • a-raf
  • araf
  • araf1
  • pks2
  • rafa1
  • A-RAF
  • ARAF1
  • PKS2
  • RAFA1
  • 1200013E08Rik
  • A-Raf
  • AW495444
  • Araf1
Alternatives
anti-Human ARAF antibody for Western Blotting
Epitope
AA 183-199
21
20
18
15
14
13
13
9
8
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
127
67
62
3
3
2
1
1
Host
Mouse
108
21
Clonality (Clone)
Monoclonal ()
Conjugate
This ARAF antibody is un-conjugated
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
83
52
34
26
19
9
3
2
2
1
1
1
1
1
Supplier
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Immunogen Mouse A-Raf aa. 183-199
Clone 1-A
Isotype IgG1
Cross-Reactivity Human, Chicken, Dog (Canine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name A-Raf (ARAF Antibody Abstract)
Background A-Raf, a proto-oncogene that encodes a 68 kDa serine/threonine-specific protein kinase, shows strong homology to c-Raf and v-Raf in the kinase region of the molecule. c-Raf appears to be uniformly and ubiquitously expressed, but A-Raf expression is much more variable. A-Raf is found at highest levels in urogenital and kidney tissues, with the lowest levels found in brain. Cell transformation by A-Raf has been shown to be independent of p21[ras], suggesting the possibility of an independent signal transduction pathway. This antibody is routinely tested by western blot analysis.
Molecular Weight 68 kDa
Pathways MAPK Signaling, RTK Signaling
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (AA 183-199) (ABIN967727) Western blot analysis of A-Raf on SKN, a human neuroblastoma, cell lysate. Lane 1: 1:...
 image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (AA 183-199) (ABIN967727) Immunoflourescence staining of rabbit kidney.
 image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (AA 183-199) (ABIN967727) anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody (Image 3)
Western Blotting (WB) image for anti-ARAF antibody (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog) (AA 183-199) (ABIN967727) anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 183-199) antibody (Image 4)
Product cited in: Gomez, Pritchard, Herbert: "cAMP-dependent protein kinase and Ca2+ influx through L-type voltage-gated calcium channels mediate Raf-independent activation of extracellular regulated kinase in response to glucagon-like peptide-1 in pancreatic beta-cells." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48146-51, 2002 (PubMed).

Huleihel, Goldsborough, Cleveland et al.: "Characterization of murine A-raf, a new oncogene related to the v-raf oncogene." in: Molecular and cellular biology, Vol. 6, Issue 7, pp. 2655-62, 1987 (PubMed).

Rapp, Heidecker, Huleihel et al.: "raf family serine/threonine protein kinases in mitogen signal transduction." in: Cold Spring Harbor symposia on quantitative biology, Vol. 53 Pt 1, pp. 173-84, 1989 (PubMed).

Storm, Cleveland, Rapp: "Expression of raf family proto-oncogenes in normal mouse tissues." in: Oncogene, Vol. 5, Issue 3, pp. 345-51, 1990 (PubMed).

Background publications Raabe, Rapp: "KSR--a regulator and scaffold protein of the MAPK pathway." in: Science's STKE : signal transduction knowledge environment, Vol. 2002, Issue 136, pp. pe28, 2002 (PubMed).

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